Earthworm extract, as well extraction method and application thereof

Abstract

The invention relates to an earthworm extract, as well as an extraction method and application thereof. The extract is prepared by the following steps: (1) coarse polypeptide preparation: cutting 1kg of frozen earthworm into small pieces, washing with precooled distilled water until the small pieces are completely bloodless; mincing into paste, adding precooled homogenate, repeatedly homogenizing with a colloid mill; centrifuging the homogenate, obtaining supernate, adding 95 percent ethanol so that the final concentration of the supernate is 60 percent, standing at 4 DEG C and stirring, centrifuging after 4 hours, and obtaining the supernate; performing rotary evaporation to the supernate in vacuum to recycle ethanol until the concentrate volume is 500ml; lyophilizing the concentrate to obtain an earthworm polypeptide crude extract, and storing at the temperature of minus 20 DEG C to obtain a lyophilized product for later use; and (2) desalinating and purifying crude polypeptide to obtain a lyophilized product, namely the earthworm polypeptide. According to the earthworm polypeptide extraction method systematically established in the invention, the active earthworm polypeptide with high purity and yield can be obtained; and the earthworm polypeptide has protective effect on rat pheochromocytoma cell (PC12) damage caused by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine ions (MPP+).

Description

technical field [0001] The invention relates to an earthworm polypeptide with strong biological activity extracted from earthworm adults, and also relates to an extraction method of the earthworm polypeptide and an application of the earthworm polypeptide, belonging to the technical field of biochemistry. Background technique [0002] Parkinson's disease (Parkinson's disease, PD) is a common neurodegenerative disease in the middle-aged and elderly, and its main pathological features are the loss of dopamine (DA) neurons in the substantia nigra and the formation of Lewy bodies. With the acceleration of my country's population aging process, the incidence of PD is increasing day by day. At present, the vast majority of PD patients are mainly treated with drugs. Although it can improve clinical symptoms, long-term application will cause side effects such as "on-off" phenomenon, motor and mental disorders, and it still cannot delay the progressive degeneration of DA neurons. In...

AI Technical Summary

Problems solved by technology

As a commonly used animal medicinal material, it has a wide variety of chemical components and complex structures, and most of them are high-molecular organic compounds. Although it has been widely used due to its remarkable pharmacological activity and abundant resources, the basic research on chemical components and pharmacology is relatively low. Weakness greatly limits its in-depth application in clinical practice. As a natural source of active peptides, earthworm peptides have small molecular weight, relatively easy preparation, high purity, weak antigenicity, clear functions, strong specificity, and small toxic and side effects. , easy multi-channel absorption and other incomparable advantages of macromolecular proteins

At present, there are no reports on the extraction methods and specific pharmacological effects of earthworm polypeptides.

Images