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Optimized culturing method for promoting growth of Amanitaflavipes mycelia

A culture method and a technology for optimizing the culture medium, which are applied in the field of indoor culture of large fungi, can solve the problems of difficult induction of mycelium, slow growth of mycelium, and influence on artificial culture, etc., and achieve the effect of shortening the cycle, accelerating the growth speed, and shortening the growth cycle

Inactive Publication Date: 2012-10-03
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The artificial domestication or cultivation of Amanita is the premise or key to ensure the sustainable utilization of Amanita resources, but it is still a problem and blind spot that is difficult to overcome. Among them, it is difficult to induce mycelium, and the very slow growth of mycelium restricts and affects artificial cultivation. important link or factor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] Mature fruiting bodies picked in the field with good growth, no pests, unopened or incompletely opened umbrellas, thick stipe and thick cap;

[0021] Bring the fruiting body back to the laboratory, wipe the surface of the cap and the stipe lightly with an alcohol cotton ball on the ultra-clean table to remove the surface sediment or soil, divide the fruiting body into four parts from the middle of the cap, and dissect it Take the internal tissue block at the junction of the stipe and the cap with a knife, and cut into about 0.08-0.16cm 2 small pieces;

[0022] Gently embed the small tissue pieces cut above into the test tube slant induction medium (potato 200.00g / L, glucose 20.00g / L, NH 4 NO 3 0.30g / L, glutamic acid 0.32 g / L, V B1 0.10mg / L, wort juice 150 ml / L, agar 11.00 g / L, control the pH value to be about 5.4) on the surface, culture temperature is 21-22℃, dark culture for 14 days, very few white bacteria began to germinate around the bacteria block Silk, afte...

example 2

[0026] Mature fruiting bodies picked in the field with good growth, no pests, unopened or incompletely opened umbrellas, thick stipe and thick cap;

[0027] Bring the fruiting body back to the laboratory, wipe the surface of the cap and the stipe lightly with an alcohol cotton ball on the ultra-clean table to remove the surface sediment or soil, divide the fruiting body into four parts from the middle of the cap, and dissect it Take the internal tissue block at the junction of the stipe and the cap with a knife, and cut into about 0.08-0.16cm 2 small pieces;

[0028] Gently embed the small tissue pieces cut above into the test tube slant induction medium (potato 200.00g / L, glucose 20.00g / L, NH 4 NO 3 0.40g / L, glutamic acid 0.28g / L, V B1 0.10mg / L, wort juice 150 ml / L, agar 11.00 g / L, control the pH value to be about 5.4) on the surface, culture temperature is 21-22℃, culture in dark for 16 days, very few white bacteria begin to germinate around the bacteria block Silk, 55...

example 3

[0032] Mature fruiting bodies picked in the field with good growth, no pests, unopened or incompletely opened umbrellas, thick stipe and thick cap;

[0033] Bring the fruiting body back to the laboratory, wipe the surface of the cap and the stipe lightly with an alcohol cotton ball on the ultra-clean table to remove the surface sediment or soil, divide the fruiting body into four parts from the middle of the cap, and dissect it Take the internal tissue block at the junction of the stipe and the cap with a knife, and cut into about 0.08-0.16cm 2 small pieces;

[0034] Gently embed the small tissue pieces cut above into the test tube slant induction medium (potato 200.00g / L, glucose 20.00g / L, NH 4 NO 3 0.35g / L, glutamic acid 0.30 g / L, V B1 0.10 mg / L, wort juice 150 ml / L, agar 11.00 g / L, control the pH value to be about 5.4) on the surface, culture temperature is 21-22 ℃, dark culture for 18 days, very few white bacteria began to germinate around the bacteria block Silk, 53...

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Abstract

The method relates to a culturing method for promoting growth of Amanitaflavipes mycelia, belonging to the technical field of culturing of macro fungi.. The culturing method is characterized in that mycelia are induced with mature sporocarps without pileus and a culturing medium is improved so as to provide a simple optimized culturing medium with short culturing circle, low cost and capability of promoting the fast growth of mycelia. Amanita belongs to special and precious economical macro fungi, is high in value and wide in application range and has potential application prospect in the field of development of new specific medicines such as anti-tumor, antibacterial and antiviral, sedative or narcotic medicines, but Amanita is hard to develop and use so far because of rear resource, difficulty in artificial acclimatization, inactive chemical synthetics of toxins and other problems. According to the method, Amanitaflavipes mycelia collected from Shishan, Wuding, Yunnan is used as the raw material and innovative breakthrough is made aiming at the problems limiting the culturing, such as difficulty in mycelium induction and slow growth of mycelia, thereby providing conditions for large-scale culturing and artificial acclimatization of Amanitaflavipes mycelia in the further.

Description

technical field [0001] The present invention relates to a kind of accelerated yellow handle amanita ( Amanita flavipes ) The method for cultivating mycelium growth belongs to the field of biotechnology, and specifically belongs to the category of indoor cultivation of large fungi. Background technique [0002] Amanita ( Amanita ) belongs to Basidiomycotina, Phytomycetes, Agaricales, Amanitaceae ( Amanitaceae ), is a more special and valuable worldwide widespread genus among the poisonous macrofungi. Its species diversity is very rich. Nearly 400 species have been reported in the world, and nearly 100 species (including subspecies) , variants and variants). Most of the species in the genus Amanita belong to the famous poisonous fungus, therefore, the value of Amanita lies in its toxin. Amanita toxins can be divided into four categories, the most important and important of which are peptide toxins, which include amatoxins, phallotoxins and virotoxins three kinds. The a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 李宗菊张曦予何隽唐萍李彪周文吴鹏赵昱冯辽辽
Owner YUNNAN UNIV
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