Chemiluminescent test kit for testing activity of MGMT (O6-Methylguanine DNA-Methyltransferase) and test method

Abstract

The invention provides a chemiluminescent test kit for testing activity of MGMT (O6-Methylguanine DNA-Methyltransferase), which comprises a streptavidin magnetic bead, a biotinylated O6-benzyl guanine, an enzyme label for MGMT specific antibody anti-MGMT, a standard substance solution of the MGMT, a luminescent substrate fluid and a concentrated cleaning solution. The invention also discloses a test method for applying the test kit to MGMT. The test method comprises the following steps: firstly, extracting total protein from a sample; testing by using the test kit; and lastly analyzing a test result. The invention provides the chemiluminescent test kit and the test method for testing the activity of MGMT in human tissues or cells; the chemiluminescent test kit and the test method have the advantages of convenience and high speed in operation, high flexibility of test result, strong specificity, and the like; and the chemiluminescent test kit and the test method have wide application prospects in the aspect of clinical test for the activity of MGMT.

Description

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AI Technical Summary

Benefits of technology

This patented technology improves upon existing methods by utilizing magnets rather than regular plate carriers like standard microplates or other types of particles used during immunological assays. It allows quicker capture of biomarkers from complex mixtures without losing their activity due to diffusion processes along with faster response times compared to traditional tests such as Western blots. Additionally, this new method simplifies sample preparation procedures while maintaining accuracy over multiple analyte levels. Overall, these technical improvements make them more efficient at detecting small amounts of substances within body fluids (blood).

Problems solved by technology

Technics Problem: Current techniques for detecting specific changes made during chemical therapies like cytoreductive surgery cannot accurately determine how effective their treatments were against cancer cell growths due to lack of accurate measurement tools at home settings. Additionally, current testing protocols require multiple steps and lengthy processes with poor accuracy and potential errors associated with sample preparations.

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