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Method for detecting or performing aided detection on sorghum sudanense nationally checked variety

An auxiliary detection, Gaodancao country's technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc.

Inactive Publication Date: 2013-06-12
全国畜牧总站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Rapid detection methods for variety identification have been established in some major crops such as corn, soybean, wheat, barley, etc., but there is no related report on Sordansis. Due to the interspecific specificity of SSR primers, each plant needs to be developed. New Primers and Detection Procedures

Method used

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  • Method for detecting or performing aided detection on sorghum sudanense nationally checked variety
  • Method for detecting or performing aided detection on sorghum sudanense nationally checked variety
  • Method for detecting or performing aided detection on sorghum sudanense nationally checked variety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1. Screening of SSR primers used to detect Sorghum grass nationally approved varieties

[0090] From the 68 pairs of polymorphic primers for Sorbus dansarius recorded in the literature, 20 pairs of primers with polymorphism, stable amplification, and easy to count were screened for variety detection. The sequences of the 20 pairs of SSR primers are shown in Table 2.

[0091] Table 2 is the SSR primers used for detection

[0092]

Embodiment 2

[0093] Example 2, the application of SSR primers used to detect Sorghum dancao national examination varieties

[0094] 1. Exploration of sampling strategy

[0095] Samples of seven nationally approved varieties of Sordansis and other 4 non-nationally approved varieties of Sordansis (Jicao 3, Sweet Sorghum, Sudangrass and unknown Sorghum grass; sources and backgrounds are shown in Table 1 ) of 400 seeds each, soaked in distilled water, placed in a refrigerator at 4°C for 7 days, and then transferred to wet sand for planting;

[0096] When the seedling height is 2cm, collect 40 leaves, 60 leaves, 80 leaves, and 100 leaves of each sample; when the seedlings are 10cm, collect 3 individual leaves of each sample Samples, mixed samples of 20 leaves, stored in liquid nitrogen or directly for the next step of analysis.

[0097] 1. Extraction of total DNA

[0098] (1) Add 850 μl of 2×CTAB solution to a 2ml centrifuge tube, add 10 μl of β-mercaptoethanol at the same time, heat in a 65...

Embodiment 3

[0150] Embodiment 3, detecting whether the sample to be tested is a nationally approved variety of Sorbus dancao

[0151] The 8 samples to be tested are grass species that have been phenotyped as the following 7 nationally approved varieties of Gaodancao and Neinong No. 1 Sudangrass:

[0152] Wancao No. 2: The leaves are hypertrophic, the stalks are thick, the length is similar to that of sorghum, the grains are small, and the color is purple-brown.

[0153] Tiannong Qingsi No. 1: purple at seedling stage, flat and wide leaves, dark brown shell, red grain.

[0154] Le Shi: The leaves are long strips, the veins are white, and the grains are flat and oval.

[0155] Wancao No. 3: The plant shape is similar to that of sorghum, with large leaves and small, purple-brown seeds.

[0156] Tiannong No. 2: purple at seedling stage, black shell, red grain.

[0157] Ji Cao No. 2: purple buds, green seedlings, abundant leaves, wide leaves, waxy veins, thick stems, juicy, compact plant ty...

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PUM

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Abstract

The invention discloses a method for detecting or performing aided detection on sorghum sudanense nationally checked variety. The invention provides a primer group for detecting and performing aided detection on sorghum sudanense nationally checked variety, wherein the primer group consists of 20 primer groups comprising the primer group P01-primer group P20. The experiment proves that 20 pairs of SSR primers are screened, the pasture is subjected to polymerase chain reaction (PCR) amplification, the electrophoretogram of 20 products and the electrophoretogram of seven sorghum sudanense nationally checked varieties are subjected to band comparison, and if the electrophoretogram of at least 18 in 20 products is consistent with the band, the pasture can be judged to be in the same variety as the sorghum sudanense in a candidate mode. The sorghum sudanense is identified according to the primers and the method, and whether the pasture to be detected is really in the seven nationally checked varieties can be simply, rapidly, efficiently and accurately identified.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for detecting or assisting in detecting the nationally approved variety of Sorbus dancao. Background technique [0002] Since 2006, the Ministry of Agriculture has continuously organized grass seed quality supervision and spot checks, and found that some unscrupulous merchants, driven by profit, changed the name of grass seeds, counterfeited domestic fine varieties, or used domestic grass seeds to counterfeit imported grass seeds, disrupting the grass seed market Order, damage the rights and interests of breeders and farmers and herdsmen. Due to the lack of technical standards for the rapid identification of the authenticity of grass seeds, scientific identification results cannot be issued during the quality supervision process, illegal traders are not punished as they should be, and the rights and interests of forage species owners are not protected. Therefore, there is a...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 高秋苏红田李玉荣屠德鹏冯葆昌马金星李存福
Owner 全国畜牧总站
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