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Preparation method of anti-phytopathogen fermentation liquid of spirea endophytic fungus

A technology of plant pathogenic bacteria and endophytic fungi, applied in the field of microorganisms

Active Publication Date: 2013-08-07
安徽省公众检验研究院有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few domestic and foreign research reports on the antibacterial activity of endophytic fungi of Spiraea chinensis against plant pathogens

Method used

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  • Preparation method of anti-phytopathogen fermentation liquid of spirea endophytic fungus

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Embodiment

[0042] The endophytic fungus of Spiraea used in this embodiment is named after Alternaria XXB07 ( Alternaria sp. XXB07), preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is March 08, 2013, and the preservation number is CGMCC No.7292;

[0043] Alternaria XXB07 ( Alternaria sp. XXB07) strain is a natural microbial drug source for fermenting and obtaining new agricultural antibiotic active ingredients against plant diseases.

[0044] The concrete preparation operation steps of the antibacterial fermented liquid of endophytic fungus Spiraea are as follows:

[0045] (1) Activation of strains

[0046] Under sterile conditions, transfer the strains of endophytic fungi of Spiraea chinensis to the potato dextrose agar solid medium plate, and cultivate them at a constant temperature for 5 days at a culture temperature of 28°C±...

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Abstract

The invention relates to a preparation method of an anti-phytopathogen fermentation liquid of a spirea endophytic fungus. The spirea endophytic fungus is named Alternaria alternata XXB07 and was collected in China General Microbiological Culture Collection Center on March 8, 2013; and the collection number is CGMCC No.7292. The method comprises the following operation steps: 1. performing strain activation; 2. performing seed culture, and 3. performing fermentation culture to obtain a fermentation liquid of the spirea endophytic fungus, filtering the fermentation liquid under aseptic conditions to remove mycelia, and passing through a 0.22-micrometer filter to obtain the antimicrobial fermentation liquid of the spirea endophytic fungus. The invention confirms the preparation method of the antimicrobial fermentation liquid of the Alternaria alternata XXB07 strain having antibacterial activity; and antimicrobial tests of the antimicrobial fermentation liquid of the Alternaria alternata XXB07 discover that a fermentation liquid eluate and a mycelium extract of the strain have mycelium growth inhibition action on plant pathogenic fungi of Phytophthora capsici.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a fermentation and cultivation method, in particular to a preparation method of an antibacterial fermentation liquid of endophytic fungi of Spiraea lanceolata. Background technique [0002] With the development of society and the improvement of human's understanding of the environment and ecology, people's understanding of pesticides is getting deeper and deeper. They realize that human beings not only need pesticides, but also realize that the widespread use of synthetic pesticides will bring great disadvantages. In the context of protecting the ecological environment, biological control has sprung up, and as an important content of biological control, agricultural antibiotics have attracted more and more attention because of their incomparable advantages over synthetic pesticides. Microbial pesticides have the characteristics of environmental safety, no pollution, no resid...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01P3/00C12R1/645
Inventor 花日茂徐庆庆柏钰曾现银操海群吴祥为
Owner 安徽省公众检验研究院有限公司
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