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Serum metabonomic method for screening biomarkers of transgenic animal

A technology of transgenic animals and biomarkers, which is applied in the fields of analytical chemistry and life sciences, can solve the problems such as the lack of screening transgenic animal biomarkers, and achieve the effects of small errors, simple pretreatment process, and rapid and efficient separation.

Inactive Publication Date: 2013-09-04
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0005] There is no report on the screening of biomarkers in transgenic animals

Method used

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  • Serum metabonomic method for screening biomarkers of transgenic animal
  • Serum metabonomic method for screening biomarkers of transgenic animal
  • Serum metabonomic method for screening biomarkers of transgenic animal

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Experimental program
Comparison scheme
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Embodiment Construction

[0024] Such as figure 1 Shown, the concrete realization process of the inventive method is as follows:

[0025] 1. Serum sample collection: Blood was collected from 8 normal pigs and 6 IGF-1 transgenic pigs. The blood was placed in negative pressure tubes without heparin, and coagulated on ice for 15-20 minutes at a temperature of 4-8°C. 1. Under the condition of centrifugal force of 1600-1800g, take the supernatant after centrifuging for 15-20min. The supernatant is the required 14 serum samples; the serum samples are frozen and stored in a -80°C refrigerator for later use.

[0026] 2. Serum sample pretreatment: Add 2-4 times the volume of acetonitrile precipitated protein to the above 14 serum samples, centrifuge for 10-15min at a temperature of 2-4°C and a centrifugal force of 12000-15000g, and then The supernatant was taken to obtain 14 serum samples.

[0027] 3. Sample mass spectrometry analysis: 14 serum samples were sequentially analyzed by ultra-high performance liqu...

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Abstract

The invention relates to a serum metabonomic method for screening biomarkers of a transgenic animal. The serum metabonomic method comprises the following steps of: analyzing serums of a normal animal and the transgenic animal to obtain a metabolic fingerprint spectrum by adopting an ultra-high performance liquid chromatography-mass spectrometry technology; analyzing and comparing the difference between metabolic fingerprint spectrums of the normal animal and the transgenic animal by adopting a multivariate statistical analysis method; and screening potential biomarkers of the transgenic animal. According to the serum metabonomic method, the variation condition of metabolite of the transgenic animal are more comprehensively and synthetically embodied; and the serum metabonomic method is suitable for screening of biomarkers of transgenic animals.

Description

technical field [0001] The invention relates to the fields of analytical chemistry and life sciences, in particular to a metabolomics method for screening transgenic animal biomarkers, which is a new method for high-throughput detection of transgenic animals. Background technique [0002] Transgenic animals are individual animals obtained through gene transfer technology that integrate foreign genes. With the continuous improvement of biological science and technology, transgenic animals are flourishing with its unique and potential advantages, but there is still a lack of relevant testing systems and standards for transgenic animals in my country and even the world. Therefore, it is imperative to develop rapid, accurate, and high-throughput detection methods for transgenic animals. [0003] Metabolomics is a newly developed discipline after genomics and proteomics. It imitates the research ideas of genomics and proteomics, conducts quantitative analysis of all metabolites ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 唐湘方张宏福韦家锦
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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