Method for preparing glycan-negative ion polysaccharide compound nanoparticles from micro-emulsions

Abstract

The invention relates to the field of medicines, and particularly relates to a method for preparing glycan-negative ion polysaccharide compound nanoparticles from micro-emulsions. According to the method, chitosan and negative ion polysaccharide are subjected to electrostatic interaction in a water / oil micro-emulsion to form compound polysaccharide nanoparticles, and more specifically, firstly a chitosan micro-emulsion and a negative ion polysaccharide micro-emulsion are prepared, and then are fixed and stirred, wherein the oil phase of the chitosan micro-emulsion is limonene. The invention provides an electrostatic compound nanoparticle carrier formed by the chitosan and other natural negative ion polysaccharide, wherein the average particle size of the carrier does not exceed 300nm, the particle size distribution are uniform, and the carrier can be used for intestinal tract targeted delivery of oral protein medicines, gene medicines and food natural activity products.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for preparing chitosan-anionic polysaccharide composite nano microspheres by microemulsion. Background technique [0002] After oral administration, protein drugs are hydrolyzed by gastric acid and catalyzed by enzymes in the gastrointestinal tract, and are not easy to pass through barriers such as the gastrointestinal mucosa, resulting in extremely low bioavailability. In order to successfully deliver protein drugs orally, they must be protected against the harsh environment of the stomach, avoiding hydrolysis and enzymatic degradation of protein drugs, and at the same time, the permeability of protein drugs to the intestinal mucosa needs to be increased. [0003] Some synthetic polymers such as polylactic acid, poly-2-oxyacid, etc. are used to embed protein drugs, but the synthesis of polymers requires a harsh environment, which is likely to cause denaturation of protein drugs;...

AI Technical Summary

Problems solved by technology

[0005] At present, the preparation technology of using the electrostatic complex of chitosan and anionic polysaccharide as the carrier of oral protein drugs or other active ingredients mainly adopts the method of mixing and stirring the two solutions to prepare composite carrier particles. The defect of this method is that the obtained Large particle size (above micron), extremely wide range of particle size distribution, and irregular particle shape, thus limiting the release of protein drugs

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