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Antigen composition, preparation method and application of antigen composition and tumour vaccine

A tumor vaccine and composition technology, applied in the field of tumor vaccines, can solve the problem of not being able to prevent multiple tumors at the same time, and achieve the effect of preventing the occurrence of tumors

Inactive Publication Date: 2013-10-30
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defect that the existing tumor vaccines cannot simultaneously prevent the occurrence of multiple tumors, the present invention provides a cell membrane vesicle vaccine capable of preventing the occurrence of multiple tumors and a preparation method thereof

Method used

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  • Antigen composition, preparation method and application of antigen composition and tumour vaccine
  • Antigen composition, preparation method and application of antigen composition and tumour vaccine
  • Antigen composition, preparation method and application of antigen composition and tumour vaccine

Examples

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preparation example Construction

[0016] The invention provides a method for preparing an antigen composition, the preparation method comprising the following steps: (1) mixing soluble protein of melanoma cells and soluble protein of lung adenocarcinoma cells to obtain tumor antigen protein; or mixing melanoma cell Tumor cells and lung adenocarcinoma cells are mixed and soluble protein is extracted to obtain tumor antigen protein; (2) the tumor antigen protein is contacted with immature dendritic cells; the immature dendritic cells have CD11c negative, CD80 negative, CD86 negative, MHC class I H-2K d Negative and MHC class II I-A d Negative surface marker phenotype and antigen-presenting activity; (3) Inducing immature dendritic cells after contact with tumor antigens into mature dendritic cells; the mature dendritic cells have CD11c positive, CD80 positive, CD86 positive, MHC class I H-2K d Positive and MHC class II I-A d positive surface marker phenotype; (4) isolating the cell membrane vesicles of the ma...

Embodiment 1

[0038] In this example, the antigen composition of the present invention was prepared according to the following steps.

[0039] (1) Put melanoma cells (purchased from ATCC with product number CRL-6475) in RPMI 1640 medium (purchased from HyClone, brand SH30809.01B, and containing 10% volume of fetal bovine serum), at 37 °C and 5% by volume CO 2 Cultivate at a low concentration until the bottom of the cell flask is almost covered, remove the medium, scrape off the cells and suspend them in phosphate buffer to obtain a melanoma cell suspension (use phosphate buffer to adjust the volume of the melanoma cell suspension so that the cell concentration 5×10 6 cells / mL), the melanoma cell suspension was ultrasonically treated (relative to 1mL of ultrasonically treated liquid, the ultrasonic power was 12W; the ultrasonic frequency was 20kHz, and the ultrasonic treatment time was 180s) to break the cells, and then The material after ultrasonic treatment was centrifuged at a speed of ...

preparation Embodiment 1

[0047] The antigen composition prepared in Example 1 was mixed with an immune adjuvant (non-methylated CpG dinucleotide oligodeoxynucleotide (CpG ODN 1826, that is, the sequence is as shown in SEQ ID NO.1 (5′-TCCATGACGTTCCTGACGTT- 3′) nucleic acid, ordered from Yingwei Jieji (Shanghai) Trading Co., Ltd.) and mixed in a weight ratio of 1:1 to obtain the tumor vaccine of the present invention.

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Abstract

The invention provides a preparation method of an antigen composition. The preparation method of the antigen composition comprises the following steps of: (1) mixing soluble protein of melanoma cells with soluble protein of lung adenocarcinoma cells to obtain tumour antigen protein, or mixing melanoma cells with lung adenocarcinoma cells and extracting soluble protein, so as to obtain tumour antigen protein; (2) contacting the tumour antigen protein with immature dendritic cells; (3) inducing the immature dendritic cells contacted with the tumour antigen into mature dendritic cells; and (4) separating cell vesidcles of the mature dendritic cells. The invention also provides antigen composition obtained by adopting the preparation method of the antigen composition and an application of the antigen composition in preparation of a tumour vaccine. Besides, the invention also provides a tumour vaccine which comprises the antigen composition and immunologic adjuvant.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to an antigen composition, a preparation method of the antigen composition, an application of the antigen composition and a tumor vaccine. Background technique [0002] The current conventional cancer treatment methods, namely surgery, radiotherapy and chemotherapy, are expensive, have a long treatment cycle, and cause serious damage to the body and mind of cancer patients. Compared with traditional treatment strategies, immunotherapy has the characteristics of autologous treatment, no damage to the body, and a wide spectrum of tumor killing. It is especially suitable for removing a small amount of remaining tumor cells, especially quiescent tumor cells that are difficult to kill by radiotherapy or chemotherapy. . [0003] Cell membrane vesicles are a kind of vesicle structure produced by cells, with a diameter of 30-100nm, which has the characteristics of good stability and long...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P35/00
CPCA61K39/0011A61K2039/5154A61K2039/5158A61K35/14A61K39/385A61P35/00C12N5/0639C12N2501/052C12N2501/22C12N2501/2304
Inventor 聂广军田欣朱墨桃
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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