Silkworm pupa-worm grass culture method capable of improving yield and quality

A technology for improving yield and cultivation methods, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve problems such as long cultivation time, and achieve the effects of short grass production time, large quantity and high quality

Active Publication Date: 2013-12-11
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The traditional method of cultivating Cordyceps militaris takes a long time to cultivate, generally takes 6

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for cultivating silkworm Cordyceps militaris with improved yield and quality, comprising the following steps:

[0027] ⑴Preparation of liquid strains:

[0028] Liquid medium preparation: the following components by weight were mixed to prepare medium: 20 grams of glucose, 3 grams of potassium dihydrogen phosphate, 4 grams of magnesium sulfate, 10 grams of peptone, 0.3 grams of vitamin B1, 2 grams of yeast extract powder, water 1000ml;

[0029] Strain preparation: divide the prepared liquid medium into Erlenmeyer flasks, 220 ml each, seal, and sterilize at 130°C for 18 minutes; take out the medium after sterilization, cool to room temperature naturally, Inoculate the Cordyceps militaris strain under bacterial conditions; shake and culture in a shaker at 20°C and 120 rpm for 7 days;

[0030] ⑵Pupus disinfection:

[0031] Take the tussah silkworm chrysalis, soak it in 75% alcohol for 1 minute, take it out and put it on the ultra-clean workbench for ultraviolet ...

Embodiment 2

[0043] A method for cultivating silkworm Cordyceps militaris with improved yield and quality, comprising the following steps:

[0044] ⑴Preparation of liquid strains:

[0045] Preparation of liquid medium: Mix the following components by weight to prepare medium: 25 grams of glucose, 5 grams of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, 5 grams of peptone, 0.5 grams of vitamin B1, 5 grams of yeast extract powder, water 1000ml;

[0046] Strain preparation: divide the prepared liquid culture medium into Erlenmeyer flasks, 200 ml each, seal, and sterilize at 125°C for 25 minutes; take out the culture medium after sterilization, cool naturally to room temperature, Inoculate the Cordyceps militaris strain under bacterial conditions; shake and cultivate for 5 days at 25°C and 120 rpm in a shaker;

[0047] ⑵Pupus disinfection:

[0048] Take the tussah silkworm chrysalis, soak it in 75% alcohol for 2 minutes, take it out and put it on the ultra-clean workbench for...

Embodiment 3

[0060] A method for cultivating silkworm Cordyceps militaris with improved yield and quality, comprising the following steps:

[0061] ⑴Preparation of liquid strains:

[0062] Liquid medium preparation: the following components by weight were mixed to prepare medium: 30 grams of glucose, 1 gram of potassium dihydrogen phosphate, 3 grams of magnesium sulfate, 6 grams of peptone, 10.1 grams of vitamin B, 3 grams of yeast extract powder, 1000ml of water;

[0063] Strain preparation: Distribute the prepared liquid culture medium into Erlenmeyer flasks, 230 ml each, seal, and sterilize at 120°C for 26 minutes; take out the culture medium after sterilization, cool to room temperature naturally, Inoculate the Cordyceps militaris strain under bacterial conditions; shake and cultivate for 5 days at 26°C and 120 rpm in a shaker;

[0064] ⑵Pupus disinfection:

[0065] Take the tussah silkworm chrysalis, soak it in 75% alcohol for 2 minutes, take it out and put it on the ultra-clean wo...

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PUM

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Abstract

The invention discloses a silkworm pupa-worm grass culture method capable of improving yield and quality. The method is characterized by comprising the steps including preparation of liquid spawn, pupa sterilization, inoculation, cultivation in dark, wound treatment of pupas, cultivation in light, harvest and the like. Compared with the conventional culture method, the silkworm pupa-worm grass culture method disclosed by the invention is short in grass germination time, great in fruit body number, vigorous in growth and short in growth period, so that not only can the yield of the worm grass be increased, but also the growth period can be greatly shortened, and thus the economic benefits are greatly increased.

Description

technical field [0001] The invention relates to a method for improving the yield and quality of silkworm Cordyceps militaris. Background technique [0002] The traditional cultivation method of silkworm Cordyceps militaris takes a long time for cultivation, generally needs 60-80 days, and the number of spores is small, generally 3-5, and its yield and quality all need to be further improved. Contents of the invention [0003] The object of the present invention is to provide a method for cultivating Cordyceps militaris with simple method, short culturing time and high yield, which improves yield and quality. [0004] Technical solution of the present invention is: [0005] A method for cultivating silkworm Cordyceps militaris that improves yield and quality is characterized in that it comprises the following steps: [0006] ⑴Preparation of liquid strains: [0007] Preparation of liquid medium: Mix the following components by weight to prepare medium: 10-30 parts of gluc...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 周美红王仲礼程显好刘宇图力古尔冯培勇
Owner LUDONG UNIVERSITY
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