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Method for producing anti-apolipoprotein B (ApoB) multi-antibody serum by using Zhejiang sheep

A technology of serum and sheep, applied in the direction of serum immunoglobulin, anti-animal/human immunoglobulin, etc., can solve the problems of low yield and titer, unable to meet the needs of use, etc., and achieve high-efficiency effects

Inactive Publication Date: 2013-12-25
ZHENJIANG WANSHAN HONGBIAN AGRI PARK
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Problems solved by technology

[0002] Apolipoprotein B (ApoB) has important physiological functions in lipoprotein metabolism, is the main structural protein of low-density lipoprotein cholesterol (LDL-CHOL), and is also an important raw material for biological reagents. The existing production method adopts traditional immunological way, the yield and potency are low, and it can no longer meet the growing demand for use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] Example: 25 Hu sheep rams were used as experimental animals, healthy sheep with a body weight of about 35 kg. Before immunization, all ear tags were put on. Automatic drinking fountains, daily pasture supply, appropriate addition of concentrate, vinegar grains, bran and vitamins, etc.

[0017] Human ApoB was inoculated with 0.1ml (combined antigen 25mg) and 1.9ml sterile PBS with complete Freund's (or incomplete) adjuvant 2ml to make immunogen emulsion for later use.

[0018] The first injection of Hu sheep immunization, front and rear groin, each groin is divided into 2 areas for injection, a total of 12 injection points, subcutaneous injection, 2 injection points on both sides of the neck are injected equally.

[0019] The second injection, with an interval of ten days, is injected at 2 points in the front and rear groin, a total of 12 injection points, and 2 points on the left and right of the posterior inguinal lymph nodes.

[0020] The third injection, with an int...

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Abstract

The invention discloses a method for producing anti-apolipoprotein B (ApoB) multi-antibody serum by using a Zhejiang sheep. The method comprises the following steps: preparing immunogenic original emulsion, selecting the Zhejiang sheep to perform immunization for four times, and the like, wherein the groin and the sole of the Zhejiang sheep are selected to inject during the immunization; finally collecting the blood and separating the serum. The titer of the multi-antibody serum which is finally produced by the production method is 1:512; the multi-antibody serum has high benefit.

Description

technical field [0001] The invention relates to an antigen production method, in particular to a method for producing anti-ApoB polyantiserum by using Hu sheep and a method for using the same. Background technique [0002] Apolipoprotein B (ApoB) has important physiological functions in lipoprotein metabolism, is the main structural protein of low-density lipoprotein cholesterol (LDL-CHOL), and is also an important raw material for biological reagents. The existing production method adopts traditional immunological way, the yield and potency are low, and it has been unable to meet the growing demand for use. Contents of the invention [0003] Purpose of the invention: In order to overcome the deficiencies of the above-mentioned prior art, the present invention provides a method for producing anti-ApoB polyantiserum by using Hu sheep, which can increase the yield and titer. [0004] Technical solution: In order to solve the above-mentioned technical problems, the method fo...

Claims

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Application Information

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IPC IPC(8): C07K16/18C07K16/06
Inventor 徐志伟
Owner ZHENJIANG WANSHAN HONGBIAN AGRI PARK
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