Trichoderma aureoviride and application thereof

A technology of Trichoderma yellow-green, cucumber, applied in the direction of application, fungi, chemicals for biological control, etc.

Inactive Publication Date: 2014-01-01
赵蕾
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some studies have shown that Trichoderma has a salt-dissolving and growth-promoting effect, there are few studies on the mechanism of Trichoderma’s salt-dissolving and promoting growth

Method used

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  • Trichoderma aureoviride and application thereof
  • Trichoderma aureoviride and application thereof
  • Trichoderma aureoviride and application thereof

Examples

Experimental program
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Embodiment Construction

[0024] Isolation of Trichoderma: Weigh 1 g of rhizosphere soil, measure 90 mL of sterile water, add it into a sterile Erlenmeyer flask containing glass beads, shake and mix for 30 minutes. After taking the soil suspension for gradient dilution, draw 0.2mL on the PDA plate containing antibiotics. Cultivate at 28°C for 4-5 days. Pick the Trichoderma colony on the plate for streak separation until a single colony is isolated. And save it on the PDA slope.

[0025] Preparation of Trichoderma spore suspension: Add an appropriate amount of sterile water to the plate producing green spores, and repeatedly blow the sterile water with a pipette to mix the Trichoderma spores on the plate with sterile water. Be careful not to use too much force when blowing to prevent mycelia from mixing into the spore suspension. Draw the spore liquid in the plate and add it to the Erlenmeyer flask containing sterile water and glass beads, shake well to disperse the clumps of spores. Take a small am...

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Abstract

The invention discloses trichoderma aureoviride which can produce IAA (Indole Acetic Acid) and siderophores and is high in phosphate solubilization property. The trichoderma aureoviride is characterized by comprising applications of relieving cucumber seedling salt stress and prompting seedling grow, wherein specifically the contents of cucumber seedling chlorophyll, soluble sugar and the soluble protein are increased, the content of MDA (Methylene Aioxyamphet Amine) is reduced under the salt stress, and the phosphate solubilization capablility of the strain is one of the desalting and grow prompting mechanisms.

Description

technical field [0001] The invention relates to a strain of Trichoderma chrysogreen and application thereof. Background technique [0002] Trichoderma (Trichoderma spp.) fungi, belonging to Dikarycota, Deuteromycotina, Hyphomycetes, Polygonales, Condylosporaceae, is a class of widely distributed bio-control beneficial bacteria. According to the Bisset system, Trichoderma can be divided into 31 species, the most common species of Trichoderma are Trichoderma harzianum, Trichoderma koningii, Trichoderma viride, Trichoderma longibrachiatum) and so on. Trichoderma is widely distributed in nature and is an important part of soil microorganisms. Soil rich in organic matter, rotting wood, and plant rhizosphere are all good habitats for it. In recent years, it has been found that Trichoderma not only has an antagonistic effect on plant pathogens, but also colonizes plant roots and becomes a plant symbiont, which promotes plant root development and plant growth, and improves the abi...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/885
Inventor 赵蕾竺晓平张亚庆
Owner 赵蕾
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