Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for purifying and rejuvenating potato virus-free tissue-cultured seedlings

A technology for purifying and rejuvenating seedlings, which is applied in horticultural methods, botany equipment and methods, horticulture, etc. It can solve the problems of high production cost of virus-free original potato, high investment cost of medium, and inability to be widely used. , to achieve the effect of realizing economic recycling, enhancing drought resistance and tenacious vitality

Active Publication Date: 2016-03-23
GANSU KAIKAI AGRI TECH DEV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the virus-free original seed potato must be obtained by tissue culture. At present, the investment cost of the medium used in potato tissue culture technology is high, which seriously affects the economic benefit. Various elements, especially the expensive trace elements, caused the production cost of the original seed potato of the virus-free potato to be too high to be widely used

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for purifying and rejuvenating potato virus-free tissue-cultured seedlings
  • A method for purifying and rejuvenating potato virus-free tissue-cultured seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. The composition of the medium for potato virus-free tissue culture includes:

[0025] Compound carrageenan: 100g / L;

[0026] Macroelements: NH 4 NO 3 1.0g / L, KNO 3 1.3g / L, CaCl 2 4H 2 O0.3g / L, MgSO 4 ·7H 2 O0.2g / L, KH 2 PO 4 0.1g / L;

[0027] Trace elements: KI0.2mg / L, H 3 BO 3 1.0mg / L, MnSO 4 4H 2 O5.5mg / L, ZnSO 4 4H 2 O2.0mg / L, Na 2 MoO 4 2H 2 O0.04mg / L, CuSO 4 ·5H 2 O0.008mg / L, CoCl 2 ·6H 2 O0.008mg / L, FeSO 4 ·7H 2 O9.5mg / L, CrCl 3 ·6H 2 O0.009mg / L;

[0028] Growth regulator: sodium α-naphthalene acetate 7.5mg / L;

[0029] Organic ingredients: inositol 25mg / L, sodium benzoate 24mg / L;

[0030] Others: 30g / L sucrose, appropriate amount of deionized water.

[0031] Production of compound carrageenan: (1) Recovered chicken manure is deodorized by high-temperature drying; (2) Add appropriate amount of deionized water to carrageenan, and heat up to 70-80°C until it is completely dissolved; (3) Dry and deodorize chicken Add deionized water to ...

Embodiment 2

[0045] The composition of the medium for potato virus-free tissue culture includes:

[0046] Compound carrageenan: 110g / L;

[0047] Macroelements: NH 4 NO 3 1.1g / L, KNO 3 1.4g / L, CaCl 2 4H 2 O0.33g / L, MgSO 4 ·7H 2 O0.22g / L, KH 2 PO 4 0.11g / L;

[0048] Trace elements: KI0.22mg / L, H 3 BO 3 1.3mg / L, MnSO 4 4H 2 O5.8mg / L, ZnSO 4 4H 2 O2.2mg / L, Na 2 MoO 4 2H 2 O0.04mg / L, CuSO 4 ·5H 2 O0.009mg / L, CoCl 2 ·6H 2 O0.009mg / L, FeSO 4 ·7H 2 O9.8mg / L, CrCl 3 ·6H 2 O0.009mg / L;

[0049] Growth regulator: sodium α-naphthalene acetate 7.5mg / L;

[0050] Organic ingredients: inositol 25mg / L, sodium benzoate 24.5mg / L;

[0051] Others: 30g / L sucrose, appropriate amount of deionized water.

[0052] The preparation method of medium for potato virus-free tissue culture is the same as example 1.

[0053] Potato virus-free tissue culture seedling cultivation method is the same as example 1.

Embodiment 3

[0055] The composition of the medium for potato virus-free tissue culture includes:

[0056] Compound carrageenan: 120g / L;

[0057] Macroelements: NH 4 NO 3 1.2g / L, KNO 3 1.5g / L, CaCl 2 4H 2 O0.35g / L, MgSO 4 ·7H 2 O0.25g / L, KH 2 PO 4 0.12g / L;

[0058] Trace elements: KI0.25mg / L, H 3 BO 3 1.5mg / L, MnSO 4 4H 2 O6.0mg / L, ZnSO 4 4H 2 O2.5mg / L, Na 2 MoO 4 2H 2 O0.05mg / L, CuSO 4 ·5H 2 O0.01mg / L, CoCl 2 ·6H 2 O0.01mg / L, FeSO 4 ·7H 2 O10.0mg / L, CrCl 3 ·6H 2 O0.01mg / L;

[0059] Growth regulator: sodium α-naphthalene acetate 7.5mg / L;

[0060] Organic ingredients: inositol 25mg / L, sodium benzoate 25mg / L;

[0061] Others: 30g / L sucrose, appropriate amount of deionized water.

[0062] The preparation method of medium for potato virus-free tissue culture is the same as example 1.

[0063] Potato virus-free tissue culture seedling cultivation method is the same as example 1.

[0064] Adopt the assay method of example 1, when counting different medium element ad...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for purification and rejuvenation of potato virus-free tissue culture seedlings. The method is characterized in that a culture medium suitable for growth of potato tissue culture seedlings is prepared through an MS culture medium, composite carrageenan and alpha-pimacol, the pre-cultured virus-free tissue culture seedlings are placed in the solid culture medium and cultured for 12 days, roots and stems of the virus-free potato tissue culture seedlings are strong, and the survival rate of seedling acclimatization and transplantation is high. Compared with an existing potato tissue culture seedling medium, the culture medium has the advantages of being low in cost and good in economic benefit.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for purifying and rejuvenating potato virus-free tissue-cultured seedlings. Background technique [0002] Potato original seed detoxification can effectively increase yield and improve quality, so the detoxification technology of potato original seed has been more and more widely used. However, the virus-free original seed potato must be obtained by tissue culture. At present, the investment cost of the medium used in potato tissue culture technology is high, which seriously affects the economic benefit. All kinds of elements, especially the expensive trace elements, caused the production cost of the original seed potato of virus-free potato to be too high and could not be widely used. At the same time, the potato virus-free tissue-cultured seedlings are directly hardened and transplanted after differentiation and cultivation. The robustness and qu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李恺屠建章张才强吴成秀火有仁
Owner GANSU KAIKAI AGRI TECH DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com