Applications of arabidopis thaliana JAV1 protein and coding gene of arabidopsis thaliana JAV1 protein in regulation of plant disease resistance and insect resistance

Abstract

The invention discloses applications of arabidopsis thaliana JAV1 protein and a coding gene of arabidopsis thaliana JAV1 protein in regulation of plant disease resistance and insect resistance. The invention also provides a method used for improving plant disease resistance and insect resistance, short hairpin RNA capable of reducing JAV1 gene expression material content, small interfering RNA, an interference vector, and interference segments. It is confirmed by experiments that, compared with wild type and null-transfection vector control groups, relative expression level of T3 generation pBI121-JAV1-RNAi transfected arabidopsis thaliana is reduced significantly, leaf infected area is 0.25 to 0.27cm<2> after inoculation by botrytis cinerea, and is obviously smaller than that of wild type and null-transfection vector control groups; the weight of beet armyworm larvae which are fed with leaves of 5 weeks for 6 days increases 1.58 to 2.03mg, and is obviously lower than that of wild type and null-transfection vector control groups; and plants are capable of growing normally in 12 days when bradysia odoriphaga larvae are put into soil with the plants of 18 days. According to the applications of arabidopsis thaliana JAV1 protein and the coding gene of arabidopsis thaliana JAV1 protein, a novel approach is provided for cultivation of disease-resistant and insect-resistant plants, and in the field of agricultural production, application range is wide, and market prospect is promising.

Description

technical field

[0001] The invention relates to the application of Arabidopsis thaliana JAV1 protein and its coding gene in regulating plant disease resistance and insect resistance. Background technique

[0002] In the process of evolution, plants will form a certain degree of ecological adaptability to avoid pests or pathogenic bacteria, to avoid pests, to tolerate pests, or to resist bacteria. Plant resistance genetic engineering based on plant resistance research is a biotechnology that utilizes the principles of molecular genetics to identify and isolate resistance genes, introduce resistance genes into recipients, and obtain regenerated individuals with stable expression and inheritance of resistance. Most of the resistance genes come from natural stress-resistant organisms, or the protective genes produced by plants in adversity. Transferring these genes to varieties with poor resistance can improve stress resistance. Most of this technology is based on the overexpr...

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