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Epidermal cell enriching instrument and dynamic enriching method thereof

An epidermal cell and enrichment instrument technology, applied in cell dissociation methods, epidermal cells/skin cells, animal cells, etc., can solve the problems of long digestion time, uneven digestion, poor mixing, etc., to achieve full and reasonable utilization, Promotes wound healing and uniform digestion

Active Publication Date: 2014-07-16
ZHENDE MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of static digestion are: poor mixing between digestive enzymes and substrate, long digestion time, and uneven digestion

Method used

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  • Epidermal cell enriching instrument and dynamic enriching method thereof
  • Epidermal cell enriching instrument and dynamic enriching method thereof
  • Epidermal cell enriching instrument and dynamic enriching method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Such as Figure 1-3 As shown, an epidermal cell enrichment instrument includes a base 1, and a control panel 2 arranged on the base 1, a digestion vessel 3, a filter vessel 4, a mixing vessel 5, a control unit 6, a heater 7, a vibrator 8, Temperature sensor 9 and power supply 10 etc., wherein, control panel 2 comprises display screen 21 and button 22, in this embodiment, above-mentioned display screen 21 adopts digital tube display, is mainly used for displaying parameters such as temperature, vibration frequency, cycle time, The buttons 22 include a power-on button and a vibration-on button. The vibrator 8 is installed at the bottom of the digestion vessel 3 to cause the digestion vessel 3 to vibrate at a certain frequency; in this embodiment, the vibrator 8 adopts a vibration motor, and the speed range of the vibration motor is 1100-2500rpm. The vibrator 8 can also be replaced by other similar parts, as long as the solution in the digestion vessel 3 can vibrate or sh...

Embodiment 2

[0054] The structure of an epidermal cell enrichment instrument is the same as in Example 1, the main difference is that the filter screen is 50 mesh.

[0055] A method for dynamically enriching epidermal cells, comprising pretreatment, digestion, filtration and liquid preparation, the specific steps are:

[0056] (1) Pretreatment: take the epidermis, and make several epidermal slices on the console 13 of the epidermal cell enrichment instrument; Medium-thick skin with a thickness of 0.5mm;

[0057] (2) Digestion: Start the power supply 10 of the epidermal cell enrichment instrument, take the epidermal sheet obtained in step (1) and place it in the digestion container 3, add digestive enzymes, control the vibrator 8 and the heater 7 through the control unit 6, and perform dynamic digestion. Obtain cell suspension; in this example, every 1.5 cm 2 Add 5ml of digestive enzymes to the sliced ​​skin, the digestive enzymes are formulated from trypsin and EDTA, wherein the mass co...

Embodiment 3

[0061] The structure of an epidermal cell enrichment instrument is the same as in Example 1, the main difference is that the filter screen is 20 mesh.

[0062] A method for dynamically enriching epidermal cells, including pretreatment, digestion, filtration and liquid preparation, the specific steps are:

[0063] (1) Pretreatment: take the epidermis, and make several epidermal slices on the console 13 of the epidermal cell enrichment instrument; the epidermis taken is a tomographic slice, and the particle size of each prepared epidermal slice is 5 mm. A medium-thick skin with a thickness of 0.6mm;

[0064] (2) Digestion: Start the power supply of the epidermal cell enrichment instrument, take the epidermal sheet obtained in step (1) and place it in a digestion container, add digestive enzymes, control the vibrator 8 and heater 7 through the control unit 10, and perform dynamic digestion to obtain cells Suspension; in this example, every 2 cm 2 Add 6ml of digestive enzymes t...

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Abstract

The invention discloses an epidermal cell enrichment instrument and a dynamic enrichment method thereof, belonging to the medical field. The epidermal cell enrichment instrument includes a base, and a control panel, a digestion vessel, a filter vessel, a mixing vessel, a control unit, a heater, a vibrator, a sensor and a power supply arranged on the base, through a dynamic enrichment digestion method, On the one hand, it can quickly disperse the epidermis to obtain a high-density epidermal cell suspension. On the other hand, it can greatly reduce the concentration and amount of digestive enzymes used, and at the same time shorten the digestion time, which is conducive to maintaining cell activity and reducing cell loss. , The differentiated epidermal cells obtained from the final solution are sprayed on the wound surface, and the epidermal cells are reversely differentiated on the wound surface to form epidermal stem cells, which promotes wound healing. The skin source is fully and reasonably utilized, and the amplification factor reaches 100-120 times.

Description

technical field [0001] The invention relates to a cell enrichment instrument and an enrichment method thereof, in particular to an epidermis cell enrichment instrument and a dynamic enrichment method thereof. Background technique [0002] In recent years, the incidence of burns in my country has been 2‰~10‰ of the total population. There are nearly ten million people with burns every year, and hundreds of thousands of people need to be hospitalized. After the burns are cured, a large number of scars are often left behind. Appearance, body shape, as well as functional and psychological aspects have a negative impact. [0003] At present, there are two main types of treatment for burn wounds with a large area using limited skin sources: [0004] 1. Particle skin technology: use physical methods to disperse limited autologous skin sources into as small particles as possible, and then replant them on the patient's wound surface. This technology can expand the skin graft by 10 to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/38C12M1/36C12N5/07
CPCC12M47/04C12N5/0625C12N2509/00
Inventor 韩春茂胡信雷鲁建国韩杨
Owner ZHENDE MEDICAL CO LTD
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