Pepper hybrid seed purity testing EST-SSR (expressed sequence tag-simple sequence repeat) molecular marker and application thereof

A hybrid hybrid purity and molecular marker technology is applied in the field of pepper hybrid purity detection EST-SSR molecular markers, which can solve the problems of poor identification result accuracy, long identification cycle, time-consuming and labor-intensive, etc., and achieve labor saving, low cost, and DNA quality. less demanding effects

Active Publication Date: 2014-03-19
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the shortcomings of the traditional crop hybrid purity test method, which is easily affected by environmental conditions and cultivation measures, the identification cycle is long, the cost is high, the accuracy of the identification results is poor, and it is time-consuming and laborious.

Method used

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  • Pepper hybrid seed purity testing EST-SSR (expressed sequence tag-simple sequence repeat) molecular marker and application thereof
  • Pepper hybrid seed purity testing EST-SSR (expressed sequence tag-simple sequence repeat) molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Sow 186 seeds of 'Rela No. 1' in a nutrient bowl, and when the plants grow to 5-6 true leaves, use the improved CTAB method (Allen GC, Flores-Vergara MA, Krasynanski S, Kumar S, Thompson WF. A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nat Protoc, 2006, 1:2320–2325) to extract genomic DNA from leaves of a single plant.

[0029] (2) The PCR amplification reaction system and procedure are as follows: PCR reaction system is 10 μL, including 10×PCR Buffer 1 μL, 0.2 mM dNTPs, primer (HE4) 50 ng, 0.5 U Taq DNA polymerase, 'Ran La No. 1' genomic DNA 20 ng , make up to 10 μL with sterile ultrapure water. The amplification program was: 94°C pre-denaturation for 5 min; 94°C denaturation for 45 s, 55°C annealing for 45 s, 72°C extension for 1 min, 35 cycles; 72°C final extension for 10 min. PCR products were stored at 10°C.

[0030] (3) Detection of amplified products: 5 μL of amplified products mixed with 2 μL of loadi...

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Abstract

The invention relates to the field of molecular markers, particularly relates to a pepper hybrid seed purity testing EST-SSR (expressed sequence tag-simple sequence repeat) molecular marker and application thereof, and discloses the pepper hybrid seed purity testing EST-SSR (expressed sequence tag-simple sequence repeat) molecular marker HE4 applied to 'hot No 1' hybrid seed purity testing.

Description

technical field [0001] The invention relates to the field of molecular markers, in particular to EST-SSR molecular markers for detecting the purity of capsicum hybrids and applications thereof. Background technique [0002] Capsicum is an annual or perennial herb belonging to the genus Capsicum in the family Solanaceae. Capsicum has become an important worldwide vegetable crop due to its unique pungent taste, color and nutritional components. A large number of studies have shown that the antioxidant substances contained in pepper fruits can protect biological organisms from oxidative damage and improve the body's immunity. In addition, capsaicinoids in capsicum can accelerate the metabolism of energy and lipids and reduce the incidence of cancer cells. With the public's full understanding of the nutritional and medicinal ingredients of peppers, the cultivation area has increased year by year. [0003] Seeds are the main means of agricultural production. Seed purity is the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 刘子记曹振木杨衍刘昭华刘维侠
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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