Method for measuring hemoglobins

A hemoglobin and measurement method technology, applied in separation methods, chemical instruments and methods, biological testing, etc., can solve the problems of decreased measurement accuracy, chromatogram deformation, and greater influence of pressure values, etc.

Active Publication Date: 2014-03-19
SEKISUI MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, measurement under such high-pressure conditions may cause deformation of the chromatogram due to the large pressure load on the flow path due to slight liquid leakage or pulsating current from the connection part of the flow path, resulting in a decrease in measurement accuracy.
In addition, compared with low-pressure conditions, the conditions of high pressure are due to the existence of non-specific adsorption in the flow path including columns and filters, the switching of eluents when using multiple eluents, and the environment. Changes in temperature, etc. have a great influence on the pressure value, so the measurement accuracy may be reduced

Method used

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  • Method for measuring hemoglobins
  • Method for measuring hemoglobins
  • Method for measuring hemoglobins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] 200 g of tetraethylene glycol dimethacrylate (manufactured by Shin-Nakamura Chemical Industry Co., Ltd.), 50 g of diethylene glycol dimethacrylate (manufactured by Shin-Nakamura Chemical Industry Co., Ltd.), and tetramethylolmethane triacrylate (manufactured by Shin-Nakamura Chemical Industry Co., Ltd.) 1.0 g of benzoyl peroxide (manufactured by NACALAI TESQUE, INC.) was dissolved in 100 g of Nakamura Chemical Industry Co., Ltd. The obtained mixture was dispersed in 2 L of an aqueous solution of 5% by weight of polyvinyl alcohol (manufactured by Nippon Synthetic Chemical Industry Co., Ltd., "Gohsenol GH-20"), and heated under a nitrogen atmosphere while stirring at a rotation speed of 300 rpm using a rotating paddle. The polymerization reaction was carried out at 80° C. for 1 hour. After 1 hour, 200 mL of an aqueous solution in which 80 g of acrylamide-tert-butylsulfonic acid (manufactured by Toagosei Co., Ltd.) was dissolved was added to the reaction system, and polyme...

Embodiment 2～4

[0143]Polymerization was performed by reducing the rotation speed during stirring during the polymerization reaction in Example 1, and a column packing material having a volume average particle diameter different from that in Example 1 was prepared. The volume average particle diameter and the column pressure value were measured by the same method as in Example 1. Table 1 shows the set rotational speed during polymerization, the volume average particle diameter, the size of the column used, the column pressure value, the flow rate during the measurement, and the pressure during the measurement. In addition, these conditions and measurement results in the following examples and comparative examples are also shown in Table 1.

[0144] Using the obtained column, the normal human blood used in Example 1, the sample containing abnormal hemoglobin, and the sample containing hemoglobin A2 were measured. figure 2 , image 3 , Figure 4 Same chromatogram.

[0145] Table 1 shows th...

Embodiment 5、6

[0147] In Examples 5 and 6, a column containing a polymer having a cation exchange group on the surface of the crosslinked polymer particle and satisfying the "regular pressure value of the column" of the present invention was prepared. However, an example is shown in which hemoglobins are measured under conditions that do not comply with the "prescribed pressure value at the time of measurement" of the present invention.

[0148] Polymerization was carried out by increasing the rotation speed during stirring during the polymerization reaction in Example 1 to prepare cation-exchange particles having different volume average particle diameters. The volume average particle diameter and the column pressure value were measured by the same method as in Example 1. Table 1 shows the set rotation speed, volume average particle diameter and column pressure during polymerization.

[0149] Using the obtained column, the normal human blood used in Example 1, the sample containing abnorma...

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Abstract

The purpose of the present invention is to provide: a method for measuring hemoglobins, which can measure hemoglobins within a short time and with high accuracy; and a method for measuring hemoglobin A1c, a method for measuring hemoglobin A1c and hemoglobin F simultaneously, a method for measuring hemoglobin A1c and hemoglobin A2 simultaneously and a method for measuring hemoglobin A1c and an abnormal hemoglobin simultaneously, each of which utilizes the above-mentioned method for measuring hemoglobins. The present invention is a method for measuring hemoglobins by liquid chromatography using a column which has cation-exchangeable particles filled therein as a column filler and has a pressure value of 9.8 plus103 to 29.4 plus105 Pa inclusive when an eluent to be used for the measurement is fed therethrough at a flow rate of 1.0 mL / minute, wherein the cation-exchangeable particles comprise crosslinked polymer particles and a polymer containing a cation-exchange group and provided on the surfaces of the crosslinked polymer particles.

Description

technical field [0001] The present invention relates to a method for measuring hemoglobins using liquid chromatography. Further, the present invention relates to a method for measuring hemoglobin A1c using the method for measuring hemoglobins, a method for simultaneously measuring hemoglobin A1c and hemoglobin F, a method for simultaneously measuring hemoglobin A1c and hemoglobin A2, and a method for simultaneously measuring hemoglobin A1c and abnormal hemoglobins . Background technique [0002] The method of measuring hemoglobins by liquid chromatography can be measured in a short time and has higher precision than other measurement methods, and therefore is particularly applied to the management of the hemoglobin A1c value of diabetic patients. [0003] In the conventional method of measuring hemoglobin by liquid chromatography, in order to measure in a short time, the eluent needs to be transported at a flow rate exceeding a certain speed, or in order to maintain high pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/60
CPCG01N2030/8822B01J20/285B01J2220/58G01N2030/027B01J39/26G01N30/48B01D15/362G01N30/88G01N30/96G01N30/60G01N33/49
Inventor 大石和之
Owner SEKISUI MEDICAL CO LTD
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