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A method suitable for high-efficiency induction of adventitious buds in suspension culture of Rose Damask

A technology of suspension culture and rose, which is applied in the field of plant tissue culture, can solve the problem of low regeneration rate of Damascus rose, and achieve the effect of reducing the probability of variation and inhibiting browning

Inactive Publication Date: 2015-07-29
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problem that the regeneration rate of Damascus rose is not high, the present invention adopts suspension culture to induce callus differentiation; the purpose of the present invention is to provide a kind of method that is suitable for the suspension culture of Damascus rose to efficiently induce non-budding, which is a large-scale rapid propagation of Damascus and Laying the groundwork for genetic manipulation research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Obtaining explants

[0027] Take young leaves about 14 days old and rinse them under tap water, then sterilize the surface with 75% alcohol for 30-40 seconds, then sterilize in 0.1% mercury liter solution for 2-6 minutes, rinse with sterile water for 3-5 times, Finally, under aseptic conditions, the leaves were cut into leaves with wounds around them, about 1×1 cm in size.

[0028] (2) Induction of callus

[0029] The cut blade is inoculated on the callus induction medium, and after 3 weeks, the callus is transferred to the fresh medium for subculture, once every 2 weeks; the composition of the callus induction medium is : Add 30g·L to conventional MS medium -1 sucrose, 0.7% agar, 2mg·L -1 Glutamine, 3.0mg·L -1 2,4-D, 1.0mg·L -1 6-BA, 10mg·L -1 AgNO 3 , the pH is adjusted to 5.8-6.2;

[0030] Cultivate under the condition that the temperature is 25°C±2°C and the light culture condition is 500-2000Lx. The periphery of the leaves swells rapidly, and the callu...

Embodiment 2

[0040] (1) Obtaining of explants is the same as in Example 1.

[0041] (2) The callus induction process is the same as in Example 1. The difference between this example and Example 1 is that the composition of the callus induction medium is as follows: add 30g·L -1 sucrose, 0.7% agar, 2mg·L -1 Glutamine, 4.0mg·L -1 2,4-D, 0.5mg·L -1 6-BA, 10mg·L -1 AgNO 3 , the pH is adjusted to 5.8-6.2;

[0042] Cultivate under the condition that the temperature is 25°C±2°C and the light culture condition is 500-2000Lx. The periphery of the leaf swells, and the callus is induced, and the induction rate reaches 100%. The majority of the induced callus is yellow or green callus that is not loose in texture.

[0043] (3) The single cell dispersion method is the same as that described in Example 1.

[0044] (4) The process of inducing adventitious buds in cell suspension culture is the same as in Example 1. The difference between this example and the example is that the formation of advent...

Embodiment 3

[0048] (1) Obtaining of explants is the same as in Example 1.

[0049] (2) The callus induction process is the same as in Example 1. The difference between this example and Example 1 is that the composition of the callus induction medium is as follows: add 30g·L -1 sucrose, 0.7% agar, 2mg·L -1 Glutamine, 5.0mg·L -1 2,4-D, 0.1mg·L -1 6-BA, 10mg·L -1 AgNO 3 , the pH is adjusted to 5.8-6.2;

[0050] Cultivate under the condition that the temperature is 25°C±2°C and the light culture condition is 500Lx-2000Lx. The swelling speed around the leaves was slow, and the callus was induced, and the induction rate was 100%. Most of the induced callus were dense and hard green callus.

[0051] (3) The single cell dispersion method is the same as that described in Example 1.

[0052] (4) The process of inducing adventitious buds by cell suspension culture is the same as that in Example 1. The difference between this embodiment and Example 1 is that the formation of adventitious buds ...

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Abstract

The invention discloses a method for efficiently inducing adventitious buds during suspension culture of rosa damascena Mill.. The method is characterized in that a 1 / 2 MS liquid medium mainly added with different plant growth regulators is employed for inducing adventitious buds, a solid-liquid-solid culture system of rosa damascena Mill. is established. The callus induction rate is 100%, the adventitious bud differentiation rate is 90.9%, the rooting rate is 100% and the transplanting survival rate is 100%. The method helps to reduce the browning degree of rosa damascena Mill. callus and reduce the variation rate during culture, is simple and practicable, helps to solve the problem that the regeneration rate of rosa damascena Mill. during tissue culture is relatively low, is high in efficiency, and provides important technical foundation for tissue culture of rosa damascena Mill. and establishing of a high-frequency in-vitro regeneration system.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for improving damascus adventitious bud differentiation through suspension culture. Background technique [0002] Damask rose (Rosa damascena Mill.), Rosaceae (Rosales), Rosaceae (Rosaceae), is an important raw material for extracting rose essential oil and rose water, mainly used in medical care, high-end perfume, beauty products, food additives, tobacco and other industries . This variety of rose contains more than 300 kinds of chemical components (such as citronellol, geraniol and other hundreds of aromatic substances, organic acids and other substances that are beneficial to beauty), as well as 18 kinds of amino acids and trace elements needed by the human body. There are more than 120 effective ingredients in the human body, more than 80 more than the existing domestic rose varieties, and it is a world-recognized high-quality rose variety. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄萱冯欢易姝利刘梦昕曾洁姚静雯贾如左佳琪谢佳恒
Owner NORTHWEST UNIV
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