Kidney-nourishing and kidney-reinforcing health-care medicinal wine and preparation method thereof
A health-care medicinal wine and kidney-tonifying technology, which is applied in the preparation of alcoholic beverages, medical formulas, antipyretics, etc., can solve the problems of unfavorable dissolution of fat-soluble components, unfavorable dissolution of water-soluble components, and reduction of drug efficacy, etc., to increase the number of mice The weight of testis and epididymis, the improvement of hypoxia resistance and swimming ability, and the effect of promoting spontaneous spermatogenesis
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Embodiment 1
[0038] Embodiment 1: the preparation method of the present invention prepares kidney-nourishing and kidney-reinforcing medicinal wine
[0039] Weigh 50 grams each of wolfberry, poria cocos, chrysanthemum, sealwort, cinnamon, yam, and citrus fruit, put them into an extraction tank and add 6 liters of 35-degree high-quality white wine to soak for 2 hours, heat reflux for 2 hours, and filter out the filtrate for later use. Add 3 liters of 35-degree high-quality white wine to the medicinal residues for the second hot reflux extraction for 2 hours, filter the filtrate for later use, then add 3.2 liters of 35-degree high-quality white wine for the third hot-reflux extraction for 2 hours, filter the filtrate, and combine the filtrates for 3 times , filtered after standing overnight, and the filtrate was subpackaged to obtain kidney-tonifying health-care medicinal wine.
Embodiment 2
[0040] Example 2: Safety Evaluation Experiment
[0041] Get medicated wine 1000ml of the embodiment of the present invention 1, concentrate under reduced pressure to 50ml, as medicated wine concentrate. Take another 20 clean-grade Kunming mice, with a body weight of 20±2 grams, half male and half female, and give the medicinal wine concentrate once every morning and evening, each time at 0.2ml / 10g mouse weight, for 7 consecutive days, observe the mice There were no abnormalities in the appearance, behavior, appetite, weight, and defecation of the mice, and none of the 20 mice died, so the half lethal dose (LD50) cannot be calculated, and the maximum tolerance of oral administration can only be roughly estimated The concentration of 1:20>40ml / kg body weight, which is more than 1000 times of the clinical dosage, shows that this kidney-tonifying and health-care medicinal wine is edible and has no toxic side effects.
Embodiment 3
[0042] Embodiment 3: mouse sperm influence test
[0043] Test group:
[0044] Commercially available Sanbian wine group (product batch number 20100312), commercially available deer antler wine group (product batch number 20100619), Example 1 group, commercially available Jinjiu group (product batch number 20100821) and normal saline group.
[0045] experiment method:
[0046] Take 50 clean-grade male Kunming mice, weighing 20 ± 2 grams, and divide them into 5 groups at random, give the above-mentioned 4 groups of medicinal wine and normal saline, and re-gavage the mice with 0.1ml / 10g, once a day, for 7 consecutive days. On day 8, the animal was sacrificed by dislodging the cervical spine, and the epididymis tissue was quickly removed, weighed by an electronic balance, ground in a mortar, diluted 15 times with Ren's solution, counted the number of sperm under a microscope, and observed its activity, Survival rate, the number of sperm was converted into 10,000 / 10mg epididymis ...
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