Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for building rat islet epithelioid stem cell line

A stem cell line and stem cell technology, which can be used in the establishment of human and other mammalian islet stem cell lines, and the establishment of rat islet-like stem cell lines, which can solve problems such as immaturity

Inactive Publication Date: 2014-06-25
GUANGDONG OCEAN UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no domestic report on the establishment of rat pancreatic endothelial-like stem cell lines, and the method for establishing rat pancreatic endothelial-like stem cell lines is still immature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for building rat islet epithelioid stem cell line
  • Method for building rat islet epithelioid stem cell line
  • Method for building rat islet epithelioid stem cell line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1. Isolation and culture of primary epithelial-like stem cells from rat islets

[0055] Aseptically take pancreatic tissue from adult SD rats, cut it into 1mm3 pieces, digest with 0.1% type IV collagenase at 37°C for 25min, and stop digestion with RPMI1640+10%NBS medium. Filter with 100-mesh filter, and centrifuge (1000rpm, 5min). DTZ staining ( figure 1 ), manually pick a single islet stained with DTZ under a microscope, put it into a petri dish covered with 0.1% gelatin, add RPMI1640+10% NBS culture medium, and place it at 37°C, 5% CO 2 , in a saturated humidity incubator. After being cultured for 3-4 days, primary rat islet epithelial-like stem cells adhered to the wall ( figure 2 ).

[0056] 2. Purification of rat pancreatic endothelial-like stem cells

[0057] Open the Petri dish and place a cloning ring with a vaseline-stained bottom in the area of ​​the ISLSCs. Add 0.25% trypsin + 0.04% EDTA digestion solution to the cloning ring hole, digest at room tempe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for building a rat islet epithelioid stem cell line, and belongs to the field of animal cell (tissue) engineering. Collagenase digests pancreatic tissue of an adult rat, pancreas islet is manually chosen, RPMI1640 is cultivated by serum, rat islet primary epithelioid cells are adhered to the wall to grow, a cloning ring is screened, the epithelioid cells are purified, and trypsin digestive juice digests and goes down to the future generation, so as to build the cell line. The rat islet epithelioid stem cell line built by adopting the method has the characteristics that the cell attachment forms polygon epithelioid, is in cloned growth, and is a normal diploid cell. An octamer transcription factor 4, a paired gene cassette 4, a paired gene cassette 6, a neurogenic factor 3 and a nidogen are expressed at the protein level, and the rat islet epithelioid stem cell line has multi-differentiating potential and is free of tumorigenicity.

Description

technical field [0001] The invention relates to a method for establishing human and other mammalian islet stem cell lines, in particular to a method for establishing rat islet-like stem cell lines, and belongs to the field of animal cell (tissue) engineering. Background technique [0002] Diabetes mellitus (DM) is one of the major diseases that endanger human health. According to statistics, the number of diabetic patients in the world has reached 346 million (Am J Stem Cells.2012,1(3):196-204.), while the traditional Drugs and insulin replacement therapy can not fundamentally cure the disease. In the past ten years, with the development of transplantation technology, pancreatic islet transplantation has achieved certain curative effect in the treatment of diabetes (N Engl J Med.2000,343(4):230-238. Diabetes.2005,54(7):2060-2069. Am J Transplant. 2008,8(11):2463-2470. Immune Netw. 2013,13(6):235-239.). However, due to the shortage of donor sources, the application of islet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12R1/91
Inventor 安立龙效梅
Owner GUANGDONG OCEAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com