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Radiation mutagenesis method of fern green spheroid with 60cor rays

A technology of green spheroids and ferns, applied in the field of plant cultivation, fern green spheroids 60Cor ray radiation mutagenesis

Active Publication Date: 2016-05-18
JIANGSU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The green spheroids of ferns have not been reported for radiation mutagenesis

Method used

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  • Radiation mutagenesis method of fern green spheroid with 60cor rays
  • Radiation mutagenesis method of fern green spheroid with 60cor rays
  • Radiation mutagenesis method of fern green spheroid with 60cor rays

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Experimental program
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Effect test

Embodiment Construction

[0028] 1. Materials and methods

[0029] 1.1 Test materials

[0030] The sterile seedlings of the ferns, Pteridium aquilinum and Pteridium aquilinum, were purchased from the Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences. Using green globular bodies (GGB) induced by sterile seedlings as materials, 60 Cor rays are the radiation source.

[0031] 1.2 Medium

[0032] The basic medium consists of MS macroelements, MS trace elements (MurashigeT, SkoogF.Arevisedmediumfromrapidgrowthandbioassayswithtobaccotissuecultures[J].Physiolplant,1962,15:473~497) and B 5 Vitamins (GamborgOL, MillerRA, OjimaK. Nutrientrequirementsofsuspensionculturesofsoybeanrootcells[J].Exp.CellRes.,1968.50:151~158), a commonly used medium in plant tissue culture, sucrose 30g / L, agar strips (Chengfeng brand, Quangang Chemical Plant, Quanzhou City, Fujian) 8g / L;

[0033] Green spheroid induction and proliferation medium additional hormone: 6-benzylaminopurine (abbreviated as BAP, Shanghai X...

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Abstract

The invention relates to a pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method, and belongs to the technical field of a plant cultivation method. The pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method is characterized in that a green spheroid generated by inducing a pteridophyte aseptic seedling under the condition of isolated culture is used as a material, and <60>Cor rays are used as radiation sources; the green spheroid subjected to irradiation treatment is transferred to a grow-up seedling culture medium containing 0.2mg / L of additional indolebutyric acid (IBA) and germinates, thus a complete plantlet with roots, stems and leaves is formed; the dosage rate of the<60>Cor rays is 1Gy / min, the irradiation dose is 80Gy, and the seedling rate of the green spheroid of neocheiropteris palmatopedata is 38%; the seedling rate of the green spheroid of pteris multifida poir is 26%. The pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method provided by the invention provides a new technology for development of irradiation mutagenesis breeding of pteridophyte on the organ level and is suitable for breeding of pteridophyte new varieties.

Description

Technical field [0001] Green spheroid of fern of the present invention 60 Cor ray radiation mutagenesis method involves a method suitable for plant tissue culture and 60 Cor ray radiation mutagenesis technology belongs to the technical field of plant cultivation methods. Background technique [0002] Ferns, also called ferns, originated from naked ferns in the Silurian and Devonian periods of the Paleozoic. The taxonomic status of pteridophytes lies between bryophytes and seed plants. There are currently about 12,000 species in the world, mainly distributed in tropical and subtropical regions. There are more than 2,600 species in China (Qin Renchang, 1959, Flora of China (Volume 2) ).Beijing: Science Press). The propagation methods of ferns are divided into sexual propagation (spore propagation), asexual propagation (vegetative propagation) and tissue culture. The breeding method is introduction and domestication (Han Jing, Zhao Li. Research progress on pteridophyte propagation[...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 叶晓青佘建明邓衍明贾新平梁丽建孙晓波
Owner JIANGSU ACAD OF AGRI SCI
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