A kind of water-soluble Trichoderma granulosa melanin and its use as lead excretion drug

A technology of P. granulosa and melanin, which is applied in the field of water-soluble P. granulosa melanin and its use as a lead excretion-promoting drug, and achieves the effects of simple and economical method and no toxic and side effects.

Inactive Publication Date: 2017-01-04
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese Patent No. ZL201110072469.2 discloses the preparation of intracellular melanin by liquid fermentation of a strain of the inventor's research group whose preservation number is CCTCC No: M2011054 Trichomonas YM-292 (the number code determined by the inventor's research group) Method, the prepared intracellular melanin has a strong photoprotective effect, and it is safe and non-toxic after toxicological tests, but its solubility in water at room temperature is only 0.11g / 100g
At present, there are no public documents at home and abroad that mention the carboxymethylation of Trichomonas melanin to modify it into water-soluble melanin, and its application in the preparation of lead-expelling drugs

Method used

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  • A kind of water-soluble Trichoderma granulosa melanin and its use as lead excretion drug
  • A kind of water-soluble Trichoderma granulosa melanin and its use as lead excretion drug
  • A kind of water-soluble Trichoderma granulosa melanin and its use as lead excretion drug

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Experimental program
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Embodiment 1

[0013] 1. Preparation of intracellular melanin (LIM) of Trichomonas granulosa:

[0014] Inoculate Trichomonas granulosus with strain preservation number CCTCC No: M2011054 in a 100L fermenter, the medium formula is: tyrosine concentration 1.0mmol / L, copper ion concentration 1.1mmol / L, sucrose mass 25.5g / L, yeast extract 5.0g / L, peptone 5.0g / L, pH8.0, loading coefficient volume ratio is 0.7, inoculum size is 5% (v / v), culture temperature is 27°C, ventilation volume is 1.0L / min, the stirring speed is 180r / min, and the fermentation time is 12d; the fermentation liquid is suction-filtered, the mycelium is collected, dried at 60°C, and the mass (g) of the mycelium and the concentration of sodium hydroxide are 1mol / L (mL) ratio is 1:100, mix well, stand at 30°C for 12h, and filter to obtain the extract; add 1mol / L HCl to the above extract to adjust the pH to 1-2, and centrifuge the above suspension after standing for 12h. The obtained precipitate was washed with deionized water u...

Embodiment 2

[0056] First prepare and obtain the intracellular melanin of Trichodia granulosus according to the following conditions and methods: Inoculate the Trichodia granulosa with the strain preservation number CCTCCNo: M2011054 in a 100L fermenter, and the medium formula is: the concentration of tyrosine is 1.2mmol / L , copper ion concentration is 1.2mmol / L, and sucrose quality is 30g / L, yeast extract 5.0g / L, peptone 5.0g / L, pH8.0, and loading factor volume ratio is 0.7, and inoculum size is 7% (volume ratio ), the cultivation temperature is 25°C, the ventilation rate is 1.2L / min, the stirring speed is 180r / min, and the fermentation time is 15d; (g) mix with sodium hydroxide (mL) with a concentration of 1.5mol / L at a ratio of 1:150, extract in a water bath at 30°C for 12 hours, and filter with suction to obtain the extract; adjust the pH value of the above extract with 1mol / L HCl 2, let it stand for 20 hours to obtain a melanin suspension; then centrifuge the above melanin suspension,...

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Abstract

The invention discloses a water-soluble lachnum melanin and an application thereof in preparation of lead-removing promoting medicines. The water-soluble lachnum melanin is characterized by being prepared by modifying the intracellular melanin produced by the lachnum with the strain preservation number of CCTCC No:M2011054 via chloroacetic acid. According to the pharmacological action experiment for promotion of lead removal from a rat infected with the lead, the result shows that the water-soluble lachnum melanin has an obvious lead-removing promoting function and cannot generate toxic and side effects, so that the side effect defects that certain essential elements such as Zn, Cu, Fe and the like in vivo are lost and certain organs are damaged, which are caused by the existing western medicine during the lead removing, are overcome. Thus, the lead-removing promoting function of the water-soluble lachnum melanin is ensured. The method disclosed by the invention is simple and economical. The water-soluble lachnum melanin can be applied to the preparation of the tablet-type or capsule-type lead-removing promoting medicines for oral administration.

Description

technical field [0001] The invention belongs to the technical field of application of Lachnum melanin in the preparation of medicines, and in particular relates to the water-soluble melanin of Lachnum which is modified and modified with chloroacetic acid under the strain preservation number of CCTC CNo: M2011054. Melanin, and its use as a medicine for promoting the discharge of lead. Background technique [0002] Lead is one of the common heavy metals that threaten human life and health. With the development of the economy, lead pollution has expanded from the occupational environment to the daily life environment. Lead is toxic to various organ systems of the body, which will weaken the body's resistance, increase the body's susceptibility to bacteria and viruses, and the incidence of tumors. At present, western medicine is usually used for lead removal, and it is often taken after obvious poisoning symptoms appear, but the adverse reactions are large and it is not suitab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/00A61K36/06A61P39/02
Inventor 叶明李兰宋升珐娜
Owner HEFEI UNIV OF TECH
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