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Antimicrobial peptide electrochemiluminescence sensor and preparation method and detection method thereof

An antimicrobial peptide and electrochemical technology, which is applied in the field of biosensing technology and electrochemistry, can solve the problems of difficult online analysis, high requirements for operators, cumbersome sample pretreatment, etc., to achieve accelerated electron transfer rate and strong anti-interference ability , Realize the effect of online analysis

Active Publication Date: 2014-10-01
芒特拓普科技(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when these methods are applied, the sample pretreatment is cumbersome, the required instruments are expensive and bulky, the detection time is long, and the requirements for operators are high, so it is difficult to realize online analysis

Method used

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  • Antimicrobial peptide electrochemiluminescence sensor and preparation method and detection method thereof
  • Antimicrobial peptide electrochemiluminescence sensor and preparation method and detection method thereof
  • Antimicrobial peptide electrochemiluminescence sensor and preparation method and detection method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0062] A preparation method of an antimicrobial peptide electrochemiluminescence sensor includes the following steps:

[0063] 1. Provide a bare gold electrode, place the bare gold electrode in 5mL Cys solution (containing 0.1mol / L HCl) with a concentration of 20mmol / L, and electroplate the L-cysteine ​​layer on the surface of the bare gold electrode by cyclic voltammetry , The voltage is -0.5~1.0V, the scanning speed is 10mV / s, and the electroplating time is 20min; then soak and clean the electrode in water to remove the unreacted Cys molecules on the surface to obtain an electrode modified with L-cysteine ​​layer;

[0064] 2. Add 40μL of nano-gold dispersion with a particle size of about 10nm to the surface of the electrode modified by the L-cysteine ​​layer, and react at 4°C for 20h to prepare a nano-gold layer on the surface of the L-cysteine ​​layer To obtain an electrode modified with a nano-gold layer and an L-cysteine ​​layer;

[0065] The preparation method of nano gold dis...

Embodiment 2

[0072] A preparation method of an antimicrobial peptide electrochemiluminescence sensor includes the following steps:

[0073] 1. Provide a bare gold electrode, place the bare gold electrode in 5mL of a 15mmol / L Cys solution (containing 0.1mol / L HCl), and use cyclic voltammetry to electroplate the L-cysteine ​​layer on the surface of the bare gold electrode , The voltage is -0.5~1.0V, the scanning speed is 5mV / s, and the electroplating time is 30min; then soak and clean the electrode in water to remove the unreacted Cys molecules on the surface to obtain an electrode modified with L-cysteine ​​layer;

[0074] 2. Drop 40 μL of nano-gold dispersion with a particle size of about 10 nm on the surface of the electrode modified by the L-cysteine ​​layer, and react at 4°C for 22 hours to prepare a nano-gold layer on the surface of the L-cysteine ​​layer To obtain an electrode modified with a nano-gold layer and an L-cysteine ​​layer;

[0075] 3. Add 60 μL of cecropin B antibody solution wi...

Embodiment 3

[0078] A preparation method of an antimicrobial peptide electrochemiluminescence sensor includes the following steps:

[0079] 1. Provide a bare gold electrode, place the bare gold electrode in 5mL Cys solution (containing 0.1mol / L HCl) with a concentration of 25mmol / L, and electroplate the L-cysteine ​​layer on the surface of the bare gold electrode by cyclic voltammetry , The voltage is -0.5~1.0V, the scanning speed is 15mV / s, and the electroplating time is 25min; then soak and clean the electrode in water to remove the unreacted Cys molecules on the surface to obtain an electrode modified with L-cysteine ​​layer;

[0080] 2. Drop 40 μL of nano-gold dispersion with a particle size of about 7nm on the surface of the electrode modified by the L-cysteine ​​layer, and react at 4°C for 24 hours to prepare a nano-gold layer on the surface of the L-cysteine ​​layer To obtain an electrode modified with a nano-gold layer and an L-cysteine ​​layer;

[0081] 3. Add 40μL of cecropin B antibod...

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Abstract

The invention provides an antimicrobial peptide electrochemiluminescence sensor which comprises a bare gold electrode, and an L-cysteine layer, a nano-gold layer and an antimicrobial peptide antibody layer which are stacked on the surface of the bare gold electrode in sequence, wherein the antimicrobial peptide antibody layer and the nano-gold layer are combined together through electrostatic adsorption. The antimicrobial peptide electrochemiluminescence sensor is small in size and low in cost, and can sensitively and quickly detect antimicrobial peptide on line. The invention provides a preparation method for the antimicrobial peptide electrochemiluminescence sensor. The preparation method for the antimicrobial peptide electrochemiluminescence sensor is simple and easy to operate. The invention also provides application of the antimicrobial peptide electrochemiluminescence sensor. The electrochemiluminescence sensor is high in stability, repetitiveness and specificity.

Description

Technical field [0001] The present invention relates to the fields of biosensing technology and electrochemical technology, in particular to an antibacterial peptide electrochemiluminescence sensor and a preparation method and detection method thereof. Background technique [0002] Antimicrobial peptide is a kind of small molecule polypeptide with multiple biological activities that exists widely in organisms. It has the advantages of thermal stability, broad-spectrum antibacterial and potential anti-tumor properties, so it is widely used in clinical medicine, medicine, and biological feed. Additives, natural food preservatives and genetic engineering of animal and plant disease resistance. Antimicrobial peptide activity, mechanism of action, production of antimicrobial peptides, and quality control of antimicrobial peptide products have become hot issues in the research of antimicrobial peptides. The research on these issues urgently requires accurate, sensitive, and simple quan...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 栾崇林代建国
Owner 芒特拓普科技(深圳)有限公司
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