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A kind of preparation method of Mortierella sinensis mycelia polysaccharide

A technology of mycelium polysaccharides and Trichosinus sinensis, which is applied in the direction of antineoplastic drugs, drug combinations, allergic diseases, etc., can solve the problem of low extraction rate of polysaccharides, and achieve the effect of high yield and low cost

Active Publication Date: 2016-09-28
上海沛元农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its mycelium contains a higher content of polysaccharides, which play an important role in health food, medicine and other fields, but the current method of extracting polysaccharides from the mycelium of the bat moth Mortierella sinensis is insufficient, making the extraction rate of polysaccharides lower

Method used

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  • A kind of preparation method of Mortierella sinensis mycelia polysaccharide
  • A kind of preparation method of Mortierella sinensis mycelia polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Extraction of polysaccharides from Mortierella sinensis mycelium: Using Mortierella sinensis mycelium as raw material, the particle fineness is pulverized into ultrafine powder with a particle size of 10 μm by ultrafine pulverization. After ultrafine pulverization, 50 g of superfine Add 1000mL of distilled water to the finely pulverized powder, soak at room temperature for 60 minutes, heat to boiling, keep boiling slightly for 120 minutes, centrifuge at 8000g for 20 minutes; separate the precipitate and supernatant, take the precipitate and repeat the above steps to extract once more, that is Add distilled water to the precipitate, soak at room temperature, heat to boiling, keep slightly boiled, centrifuge, take supernatant, combine the supernatant obtained by two centrifuges, that is the crude extract of Mortierella sinensis mycelia polysaccharide.

[0021] Concentration of the crude extract of Mortierella sinensis mycelia polysaccharide: Concentrate the above crude ext...

Embodiment 2

[0025] Extraction of polysaccharides from Mortierella sinensis mycelium: Using Mortierella sinensis mycelium as raw material, the particle fineness is pulverized into an ultrafine powder with a particle size of 9 μm by ultrafine pulverization, and 50 g of superfine pulverized powder is weighed. Then add 800mL of distilled water, soak at room temperature for 40 minutes, heat to boiling, keep boiling slightly for 100 minutes, centrifuge at 8000g for 20 minutes; separate the precipitate and supernatant, take the precipitate and repeat the above steps to extract once more, that is, add distilled water to the precipitate , soaking at room temperature, heating to boiling, centrifuging after keeping slightly boiling, taking the supernatant, combining the supernatant obtained by two centrifuges, which is the crude extract of the polysaccharide of Mortierella sinensis mycelia. Concentration of the crude extract of Mortierella sinensis mycelia polysaccharide: Concentrate the above crude ...

Embodiment 3

[0027]

[0028] The above table shows the extraction rate of Mortierella sinensis mycelium polysaccharide obtained by 6 extractions by the method of the present invention and the extraction rate of Mortierella sinensis mycelium polysaccharide obtained by 6 extractions by the prior art.

[0029] Since the present invention can firstly pulverize the cell walls of the Mycelium of the Mortierella sinensis through ultrafine pulverization, and then through a series of follow-up methods, the polysaccharides in the Mycelia of the Mortierella sinensis can be more thoroughly and effectively Extraction; while the prior art does not undergo ultrafine pulverization, but only extracts by conventional methods such as simple soaking.

[0030] Because also contain trace other impurity in the Mortierella sinensis mycelia polysaccharide after extraction, so when calculating pure polysaccharide (polysaccharide described in the present invention all refers to Mortierella sinensis mycelia polysac...

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Abstract

The present invention relates to a preparation method of Mortierella sinensis mycelium polysaccharide, comprising: S1: Extraction of Mortierella sinensis mycelium polysaccharide: using Mortierella sinensis mycelium as raw material, ultrafine pulverization until the particle size is 7-11μm ultra-fine powder, added to water, soaked, then heated to boiling, kept slightly boiled, centrifuged, and the supernatant was obtained as the crude extract of Mortierella sinensis mycelia polysaccharide; S2: Concentrated to obtain a concentrated solution ; S3: precipitation: adding ethanol, stirring while adding, standing still, centrifuging, and collecting the precipitate; S4: drying: freeze-drying to obtain the polysaccharide of Mortierella sinensis mycelia. The method is simple, efficient, low in cost, high in polysaccharide yield and content, and suitable for large-scale production.

Description

technical field [0001] The invention relates to a mycelium polysaccharide in the biological field, in particular to a preparation method of Mortierella sinensis mycelium polysaccharide. Background technique [0002] Mortierella sinensis fermentation mycelium is also called Cordyceps fermented mycelium Cs-C-Q80 or mycelium of Mortierella chinensis, which is a true Cordyceps sinensis fungus. Its mycelium contains a high content of polysaccharides, which play an important role in health food, medicine and other fields, but the current method of extracting polysaccharides from the mycelium of the bat moth Mortierella sinensis is insufficient, making the extraction rate of polysaccharides lower. Contents of the invention [0003] The purpose of the present invention is to provide a preparation method of Mortierella sinensis mycelium polysaccharide, which is a simple, efficient, low-cost, high polysaccharide yield and content, and suitable for large-scale production, which over...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P37/04A61P35/00
Inventor 庞小博史先敏
Owner 上海沛元农业发展有限公司
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