Method for quickly determining light intensity and temperature adaptation range of plants and device thereof

A technology that adapts to the scope and plants, applied in the field of plant environmental stress, can solve the problems of photosynthetic mechanism damage, poor plant growth, death and other problems, and achieve the effect of light intensity

Inactive Publication Date: 2014-12-24
NANJING FORESTRY UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

Plants growing in the above-mentioned environment for a long time will inevitably cause damage to the photosynthetic mechanism, poor growth or even death of the plants

Method used

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  • Method for quickly determining light intensity and temperature adaptation range of plants and device thereof
  • Method for quickly determining light intensity and temperature adaptation range of plants and device thereof
  • Method for quickly determining light intensity and temperature adaptation range of plants and device thereof

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Embodiment Construction

[0039] The present invention will be further described below in conjunction with the accompanying drawings. The following examples are only used to illustrate the technical solution of the present invention more clearly, but not to limit the protection scope of the present invention.

[0040] A method for quickly determining the light intensity and temperature adaptation range of plants, comprising the steps of:

[0041] (1) Collection of samples to be tested:

[0042] In April and July, the young leaves of healthy Ginkgo biloba plants with yellow leaves and green leaves were collected, and a leaf disc with a diameter of 1 cm was cut from each leaf with a puncher, and placed in a light-proof box at 4°C save.

[0043] (2) Selection of processing conditions and time of the sample to be tested:

[0044] According to the monthly average and extreme temperature values ​​of the ginkgo planting site (Nanjing) in April and July, set the following four temperature gradients: 26°C, 2...

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Abstract

The invention discloses a method for quickly determining the light intensity and temperature adaptation range of plants. The method comprises, based on treatment of different temperatures and different light intensities on detached leaves and subsequent dark recovery for a certain time, dynamically monitoring the Fv/Fm fluorescent parameters of the leaves; determining the temperature range of irreversible optical damage of the plants by drawing an Fv/Fm fluorescent kinetic curve under treatment conditions and analyzing the descending ratio of Fv/Fm after samples are processed and the rising ratio of Fv/Fm after the samples are restored. The method for quickly determining the light intensity and temperature adaptation range of the plants avoids damage to growth of the plants and facilitates parallel detection of optical damage and restoration conditions of the leaves of the plants under different temperatures and different light intensities and further determines the light intensity and temperature adaptation range of the plant and judges the resistance differences of different plants. The invention also discloses a device for achieving the method for quickly determining the light intensity and temperature adaptation range of the plants. The device for achieving the method for quickly determining the light intensity and temperature adaptation range of the plants achieve control to light intensity by changing the distance between a light source and the samples to achieve continuous light intensity control and meanwhile to guarantee constantly unchanged light quality when changing the light intensity.

Description

technical field [0001] The invention relates to a method and a device for quickly determining the range of light intensity and temperature adaptation of a plant, in particular to a method based on chlorophyll fluorescence (F v / F m ) The method for determining the extreme light intensity and temperature of a plant by measuring the parameters belongs to the technical field of plant environmental stress. Background technique [0002] The growth and development of plants depends on conditions such as light, temperature, water and soil in the outside world. However, compared to water and soil factors, the response of plant leaves to light and temperature is the most direct and sensitive. Illumination is the source of energy in the process of plant photoreaction, which starts the process of plant photosynthesis. Temperature determines the activity of related enzymes in light and reaction processes, and is the main limiting factor in dark reaction processes. Appropriate temper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/28
Inventor 曹福亮王欢利郁万文张往祥汪贵斌刘新亮
Owner NANJING FORESTRY UNIV
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