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Amino acid sequence and application of annexin A1 antigen epitope for detection of breast cancer marker

An antigenic epitope, breast cancer technology, applied in the field of immunology, can solve the problems of cell migration and invasion ability decline, and achieve the effect of high specificity and high sensitivity

Inactive Publication Date: 2017-04-12
李光辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recent studies have shown that Annexin A1 is highly expressed in high-metastasis and hormone-sensitive cell lines of breast cancer, and interference with the expression of Annexin A1 in these cells will lead to a decrease in cell migration and invasion

Method used

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  • Amino acid sequence and application of annexin A1 antigen epitope for detection of breast cancer marker
  • Amino acid sequence and application of annexin A1 antigen epitope for detection of breast cancer marker
  • Amino acid sequence and application of annexin A1 antigen epitope for detection of breast cancer marker

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Experimental program
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Effect test

Embodiment 1

[0029] Kit preparation

[0030] 1 Reagents See Tab.2-7 for reagent preparation.

[0031]

[0032]

[0033]

[0034]

[0035]

[0036]

[0037]

[0038] 2 operations

[0039] (1) Coating: The microplate should be washed 3 times with washing buffer, the working antigen should be diluted to the working concentration with the coating solution, and coated on the microplate, overnight at 4°C.

[0040](2) Add glutamic acid: wash 3 times with washing buffer, dilute glutamic acid with coating solution to a concentration of 100 μg / ml, 200 μl per well, incubate at 37°C or room temperature for 1 hour;

[0041] (3) Add plasma and quality control control (primary antibody): wash the ELISA plate three times with washing buffer, and dilute the plasma to an appropriate concentration with coating solution, generally 1:100-1:500, 100 μl per well, Incubate at 37°C or room temperature for 1 hour;

[0042] (4) Secondary antibody incubation: wash with washing buffer 3 times, ...

Embodiment 2

[0046] Detection of AnnexinA1 Auto IgG Antibody in Patients with Breast Cancer

[0047] 1 Sample collection: In this study, 311 breast cancer samples diagnosed by radiological examination and histological examination were selected from the Second Hospital of Jilin University and Provincial Cancer Hospital. All serum samples had not undergone any anti-cancer treatment before collection, and had comprehensive clinical data and information. At the same time, 303 healthy control samples were recruited. Clinical interviews and imaging examinations ruled out the possibility of breast cancer. The healthy group and the breast cancer group were comparable in gender and age (P>0.05)

[0048] 2 Test results: Annexin A1 autoantibody expression level (Tab.8): There was a statistical difference between the breast cancer group and the healthy control group (t=-7.163, P<0.001).

[0049] ROC curve analysis: the area under the ROC curve for Annexin A1 auto IgG antibody detection in breast ca...

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Abstract

The invention relates to an amino acid sequence for detecting a breast cancer marker Annexin Al antigen epitope and application of the amino acid sequence, and belongs to the technical field of immunology. The invention provides the Annexin Al antigen amino acid sequence of a related breast cancer gene. An Annexin Al polypeptide antigen is applied to detect the corresponding specific autoantibody contained in the blood of a breast cancer patient, and the autoantibody can be used as the breast cancer marker for evaluating the risk degree of breast cancer occurrence; and the antigen polypeptide and the antibody thereof can be used for preparing a breast cancer early-diagnosis reagent and developing a targeted drug for treating breast cancer.

Description

technical field [0001] The invention belongs to the technical field of immunology, and relates to the preparation of tumor early diagnosis reagents and the development of targeted drugs for treating tumors. Background technique [0002] A large number of studies have shown that tumor-associated antigens in serum or plasma can induce the body to produce autoantibodies, and there are both tumor antigens and autoantibodies against the tumor antigens in the serum of cancer patients. Therefore, both antibodies can be used to detect tumor antigens, and antigens can also be used to detect tumor antigen autoantibodies, but the specificity and sensitivity of using tumor autoantibodies to detect tumors are much higher than using tumor antigens to detect tumors. Many tumor-associated antigens exist not only in tumor patients, but also in normal people, so the detection of tumor-associated antigens is not reliable as a basis for diagnosis. However, the content of tumor autoantibodies i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47G01N33/68G01N33/574
CPCC07K14/705G01N33/57415G01N33/68G01N2333/705
Inventor 孙世龙李光辉尉军
Owner 李光辉