Method applied to quantitative detection of carbon dioxide exhaled by cancer cells
A technology for quantitative detection of carbon dioxide, applied in the field of fluorescent biosensors, can solve problems such as the lack of quantitative detection of cellular carbon dioxide and the inability to monitor the metabolic process of cancer cells
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[0042] Example 1
[0043] (1) Dissolve 0.64 mg TPP-DMAE in 0.01 mL dimethyl sulfoxide (DMSO) to obtain a concentration of 1×10 -2 mol / L solution a; add 10mL high-sugar DMEM medium to solution a to obtain a concentration of 1×10 -5 mol / L solution b.
[0044] (2) ①In order to detect whether TPP-DMAE will cause Hela cell and MCF-7 cell inactivation within the test time range, the following toxicity test experiments are done:
[0045] Pass the cervical cancer cells (Hela cell) and breast cancer cells (MCF-7cell) into two special culture dishes for confocal laser microscopes (confocal) respectively; place the Hela cell, 80-90% of the bottom of the culture dish, MCF-7cell was digested with 0.05% trypsin to make 50-60% of the cells detached from the bottom of the culture dish. Add 3 mL of high glucose DMEM medium to the culture dishes of Hela cell and MCF-7cell to stop the digestion, and then separate Hela Cell and MCF-7cell suspension were transferred from the culture dish to two 15mL cen...
Example Embodiment
[0054] Example 2
[0055] (1) Dissolve 0.64 mg TPP-DMAE in 0.01 mL dimethyl sulfoxide (DMSO) to obtain a concentration of 1×10 -2 mol / L solution a; add 10mL high-sugar DMEM medium to solution a to obtain a concentration of 1×10 -5 mol / L solution b.
[0056] (2) ①In order to detect whether TPP-DMAE will cause Hela cell and MCF-7 cell inactivation within the test time range, the following toxicity test experiments are done:
[0057] Pass the cervical cancer cells (Hela cell) and breast cancer cells (MCF-7cell) into two special culture dishes for confocal laser microscopes (confocal) respectively; place the Hela cell, 80-90% of the bottom of the culture dish, MCF-7cell was digested with 0.05% trypsin to make 50-60% of the cells detached from the bottom of the culture dish. Add 3 mL of high glucose DMEM medium to the culture dishes of Hela cell and MCF-7cell to stop the digestion, and then separate Hela Cell and MCF-7cell suspension were transferred from the culture dish to two 15mL cen...
Example Embodiment
[0066] Example 3
[0067] (1) Dissolve 0.64 mg TPP-DMAE in 0.01 mL dimethyl sulfoxide (DMSO) to obtain a concentration of 1×10 -2 mol / L solution a; add 10mL high-sugar DMEM medium to solution a to obtain a concentration of 1×10 -5 mol / L solution b.
[0068] (2) ①In order to detect whether TPP-DMAE will cause Hela cell and MCF-7 cell inactivation within the test time range, the following toxicity test experiments are done:
[0069] Pass the cervical cancer cells (Hela cell) and breast cancer cells (MCF-7cell) into two special culture dishes for confocal laser microscopes (confocal) respectively; place the Hela cell, 80-90% of the bottom of the culture dish, MCF-7cell was digested with 0.05% trypsin to make 50-60% of the cells detached from the bottom of the culture dish. Add 3 mL of high glucose DMEM medium to the culture dishes of Hela cell and MCF-7cell to stop the digestion, and then separate Hela Cell and MCF-7cell suspension were transferred from the culture dish to two 15mL cen...
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