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Method applied to diagnosis of seedling stage of gray mold

A diagnostic method and technology for botrytis, applied in the direction of material inspection products, biological testing, etc., can solve the problems of no unified identification method and evaluation system, and achieve the effects of being conducive to popularization and application, accurate results, and easy to obtain

Inactive Publication Date: 2015-02-04
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no unified identification method and evaluation system

Method used

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  • Method applied to diagnosis of seedling stage of gray mold
  • Method applied to diagnosis of seedling stage of gray mold
  • Method applied to diagnosis of seedling stage of gray mold

Examples

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Embodiment 1

[0028] 1 Seedling cultivation

[0029] Sow the layered tomato seeds into nutrient pots (height 12cm×diameter 12cm), cultivate on sand base, 20 plants per pot, 5 pots in total, the cultivation conditions are: 26°C dark and constant temperature environment; when the seedlings grow 2 true leaves At the same time, spray 1 / 2 Dutch greenhouse tomato nutrient solution, spray once every 4 days, and replenish with water during the period.

[0030] 2 Treatments under different growth conditions

[0031] When the seedling grows to 8 true leaves, select uniform seedlings and move them to 1 / 2 Dutch greenhouse tomato nutrient solution hydroponic pots (length 15cm × width 10cm × height 12cm), plant 10 plants in each pot, 5 pots in total, Arrange randomly. The air pump is normally ventilated (20min / h), and the nutrient solution is replaced every 7 days (that is, 1 / 2 Dutch greenhouse tomato nutrient solution, when replacing, all the original nutrient solution is poured out and a new nutrient...

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Abstract

The invention provides a method applied to diagnosis of the seedling stage of gray mold. The method comprises the following steps: detecting the content of ROS and calcium in leaves in the seedling stage according to disease index survey and analysis and determining standard indexes of gray mold infection as follows: ROS dyeing ratio is 0.53, and the content of calcium ions in the leaves is 139.45 micrograms per gram; in the actual diagnosis detection, when the ROS dyeing ratio is greater than or equal to 0.53 and / or the content of the calcium is greater than or equal to 140 micrograms per gram, the gray mold is avoided; when the ROS dyeing ratio is less than 0.53 and / or the content of the calcium is less than 140 micrograms per gram, the content of the calcium ions in the leaves can be regulated by taking a measure of applying fertilizer. The method has the characteristics of being simple to operate, good in repeatability and accurate in result; by virtue of the method, the method applied to diagnosis of the seedling stage of the disease is implemented; scientific basis and methods are provided for taking effective prevention measures in advance.

Description

technical field [0001] The invention belongs to the technical field of plant disease prevention and control, and in particular relates to a seedling stage diagnosis method for botrytis cinerea. Background technique [0002] Botrytis cinerea Pers. is caused by the infection of the genus Botrytis cinerea Pers., which has a wide range of hosts and can infect more than 200 species of plants such as Solanaceae, Cucurbitaceae, Rosaceae, and Fabaceae. Botrytis cinerea is a devastating disease, especially serious in tomato facilities. The yield loss caused by botrytis cinerea is generally 10% to 20%, and in severe cases it can reach more than 60%. The tomato shed destruction event caused by it. [0003] In the early stage of the disease, a series of physiological changes will occur in the leaves of the crop, and then it can infect leaves, stems, flowers and other organs; [0004] The pathogenic bacteria reproduce rapidly, mutate frequently, and are prone to drug resistance. At the...

Claims

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Application Information

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IPC IPC(8): G01N33/48
Inventor 余慧芸
Owner CHINA AGRI UNIV
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