Method for storing mosquito-eradication fungus pythium guiyangense su for long time

A kind of Pythium guiyang, long-term preservation technology, applied in microorganism-based methods, biochemical equipment and methods, preservation of microorganisms and other directions, can solve the problems of polluted environment, small environmental pollution, endangering human health and survival, etc.

Inactive Publication Date: 2015-03-25
敖云霞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Mosquitoes are one of the important medical insects. They are the vectors of various pathogens and seriously endanger the health of humans and animals. At present, chemical insecticides are mainly used to control mosquitoes. Uncontrolled use of chemical insecticides in large areas will seriously pollute the environment and destroy the ecological environment. Harmful to human health and survival, biological control is the use of various natural enemies of pests (parasites, predators, pathogenic microorganisms, etc.) to control pests at a tolerable level, which is characterized by less environmental pollution and less possibility of pest resistance , strong specificity to diseases and insect pests, relatively safe to non-target organisms, low production and marketing costs, etc., so biological control methods are gradually recognized and valued by people. The effective shelf life of fungi is very important for its development and application. The famous mosquito-killing fungi After Streptchytrium was registered and listed in the United States, commercial development was not successful because it could not be stored effectively for a long time and did not reach a certain shelf life.

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  • Method for storing mosquito-eradication fungus pythium guiyangense su for long time
  • Method for storing mosquito-eradication fungus pythium guiyangense su for long time
  • Method for storing mosquito-eradication fungus pythium guiyangense su for long time

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Experimental program
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Embodiment Construction

[0013] The present invention will be further described in detail below in conjunction with the accompanying drawings, but not as any limitation to the present invention.

[0014] Embodiments of the invention:

[0015] 1 Materials and methods

[0016] 1.1 Materials

[0017] 1.1.1 Bacteria

[0018] Pythium guiyang was isolated from the soil of Guiyang Medical College in 1994. After purification, it was inoculated in sunflower seed extract (SFE)[5] medium and KPYG2 medium. Rejuvenate and preserve the species once a month, and cultivate at a constant temperature of (25±1)°C.

[0019] 1.1.2 Mosquitoes

[0020] The mosquitoes used in this experiment were the late 2nd or early 3rd instar larvae of Culex quinquefasci-atus bred in the biological mosquito control laboratory after domestication. The breeding temperature is (25±1)°C, the relative humidity is 60%-70%, and the photoperiod is 14h:10h (light:dark).

[0021] 1.2 Method

[0022] 1.2.1 Medium

[0023] KPYG2 medium, SFE m...

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Abstract

The invention discloses a method for storing mosquito-eradication fungus pythium guiyangense su for a long time. Hyphae are stored on an agar culture-medium or a sunflower seed extract; the culture temperature range is 3 DEG C-5 DEG C; mycelia cannot survive under the condition of minus 25 DEG C and minus 75 DEG C; the oxygen partial pressure is 33.3%. According to the invention, factors which greatly affect fungus storage are summarized, and valuable data are provided for commercial development and industrialized production.

Description

technical field [0001] The invention relates to a method for long-term preservation of mosquito-killing fungus Pythium Guiyang, belonging to the technical field of medicine preparation. Background technique [0002] Mosquitoes are one of the important medical insects. They are the vectors of various pathogens and seriously endanger the health of humans and animals. At present, chemical insecticides are mainly used to control mosquitoes. Uncontrolled use of chemical insecticides in large areas will seriously pollute the environment and destroy the ecological environment. Harmful to human health and survival, biological control is the use of various natural enemies of pests (parasites, predators, pathogenic microorganisms, etc.) to control pests at a tolerable level, which is characterized by less environmental pollution and less possibility of pest resistance , strong specificity to diseases and insect pests, relatively safe to non-target organisms, low production and marketi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/645
CPCC12N1/04
Inventor 敖云霞
Owner 敖云霞
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