Biological mosquitocide and preparation method thereof

A mosquito killer and biological technology, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of not obvious mosquito killing effect, slow mosquito killing process, unacceptable and difficult to achieve drug resistance , reduce the cost of mosquito control, and have a stable effect

Active Publication Date: 2013-02-06
郑文定 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mosquito killing process of Pg is relatively slow, and the mosquito killing effect is not obviou

Method used

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  • Biological mosquitocide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A preparation method of a biological mosquito killer includes the following steps:

[0037] (1) Use SFE or KPYG2 liquid culture medium to culture Pg on a shaker for 5 days, culture temperature 25℃±1℃, shaker speed 120rpm; filter out the hyphae, wash with sterile water 3 times, add water to the original volume, and crush with a crusher 10 seconds, standby;

[0038] (2) Prepare 1%~3% (W / V) sodium alginate solution;

[0039] (3) Mix the Pg mycelium suspension with the sodium alginate solution in a ratio of 1:4, and mix well;

[0040] (4) Slowly drip the above-mentioned mixture into 1%~5%(W / V) calcium chloride solution, microcapsule particles are formed and precipitated. After 0.5~3 hours, the microcapsules are filtered out and rinsed with water for later use;

[0041] (5) Inoculate Bti bacteria in Bti liquid medium, culture on a shaker, rotate at 120rpm, temperature 30℃±1℃; after 48 hours, collect spores by centrifugation, and dilute to the following density when used: 1.4×10 5 , G...

Embodiment 2

[0048] A preparation method of a biological mosquito killer includes the following steps:

[0049] (1) Use SFE or KPYG2 liquid culture medium to culture Pg on a shaker for 5 days, culture temperature 25℃±1℃, shaker rotation speed 120rpm; filter out the hyphae, wash with sterile water 3 times, add water to the original volume, high speed grinder Crush for 10 seconds, standby;

[0050] (2) Prepare 1%~3% (W / V) sodium alginate solution;

[0051] (3) Mix the Pg mycelium suspension with the sodium alginate solution in a ratio of 1:4, and mix well;

[0052] (4) Slowly drip the above-mentioned mixture into 1%~5%(W / V) calcium chloride solution, microcapsule particles are formed and precipitated. After 0.5~3 hours, the microcapsules are filtered out and rinsed with water for later use;

[0053] (5) Inoculate Bti bacteria in Bti liquid medium, culture on a shaker, rotate at 120rpm, temperature 30℃±1℃; after 48 hours, collect spores by centrifugation, and dilute to the following density when used:...

Embodiment 3

[0059] (1) Use SFE or KPYG2 liquid culture medium to culture Pg on a shaker for 5 days, culture temperature 25℃±1℃, shaker speed 120rpm; filter out the hyphae, wash with sterile water 3 times, add water to the original volume, and crush with a crusher 10 seconds, standby;

[0060] (2) Prepare 1%~3% (W / V) sodium alginate solution;

[0061] (3) Mix the Pg mycelium suspension with the sodium alginate solution in a ratio of 1:4, and mix well;

[0062] (4) Slowly drip the above-mentioned mixture into 1%~5%(W / V) calcium chloride solution, microcapsule particles are formed and precipitated. After 0.5~3 hours, the microcapsules are filtered out and rinsed with water for later use;

[0063] (5) Inoculate Bti bacteria in Bti liquid medium, culture on a shaker, rotate at 120rpm, temperature 30℃±1℃; after 48 hours, collect spores by centrifugation, and dilute to the following density when used: 1.4×10 9 , Get the spore suspension;

[0064] (6) Mix the spore suspension and the Pg microcapsules at t...

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Abstract

The invention discloses a biological mosquitocide and a preparation method thereof. The biological mosquitocide is prepared by compounding mosquito-killing funguses and mosquito-killing bacteria; and on the basis of the mosquito-killing funguses in 1g of wet hypha, mosquito-killing funguses, i.e., bacillus thuringiensis subsp. israelensis with the number of 1.4*10<4>-1.4*10 <18> or mosquito-killing bacteria, i.e., bacilluss phaericu with the number of 2.5*10<4>-2.5*10<20> are provided. The mosquito-killing fungus is Pythium Guiyang nse Su or Pythium caronlinianum Matthews. The biological mosquitocide has the effects of killing mosquitoes fast and controlling mosquitoes in a lasting form.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a biological mosquito killer and a preparation method of the biological mosquito killer. [0002] Background technique [0003] Agricultural pests and forestry pests bring serious losses to human agriculture and forestry production every year. At the same time, mosquitoes not only harass the crowd, but also can spread more than 20 kinds of diseases, threatening human health. Thanks to the invention of chemical pesticides, people have regained food from insects, controlled the number of vector insects, and reduced the prevalence of insect-borne diseases. [0004] However, the long-term and extensive use of chemical pesticides has caused serious problems. 1) Environmental pollution, which is harmful to human and biological health. Heavy metals in pesticides remain in the soil, causing soil deterioration and reduced agricultural production; agricultural products contain high levels ...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N63/02A01P7/04
Inventor 苏晓庆
Owner 郑文定
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