Lyophyllum fumosurn molecular identification and strain production method

Abstract

The invention discloses a lyophyllum fumosurn molecular identification and strain production method. The method comprises the first step of molecular identification: ITS amplification, product sequencing, Blast isogeny data comparison and classification status determination are carried out; the second step of mother strains: a coconut juice and PDA media component is obtained according to the influence test to the lyophyllum fumosurn mother strain mycelial growth from an exogenous additive and the influence test to the lyophyllum fumosurn mother strain mycelial growth from culture conditions; the third step of stock strain: the liquid media component is screened and studied, the kernel dipping juice, 200 g of potato, 20 g of glucose, 0.3 g of monopotassium phosphate, 0.15 g of magnesium sulfate and 3 g of beef extract are obtained; the fourth step of strain cultivation; the fifth step of the strain production technology; the sixth step of the fruiting media component. The method solves the problem that in the lyophyllum fumosurn strain production process, the contaminate rate is high due to long cultivation time, and therefore the biology converstion rate of the lyophyllum fumosurn is increased.

Description

technical field [0001] The invention belongs to the technical field of edible fungus classification and strain production technology, and relates to a method for molecular identification and strain production of Binwang mushroom. Background technique [0002] The classification of present stage king mushroom is only limited to identification from form and chemical reaction, and conclusion is that king mushroom belongs to Agaricaceae, white mushroom family, and the smoke color of lily mushroom is separated from the genus, and the present invention finds that king mushroom Not only the smoke-colored Limejime, but also the lotus-leaved Limejime, which clarified the taxonomic status of Binwang mushroom; the parent species mycelia of the prior art is overgrown with a 18×180mm test tube slant culture medium, which requires 15~ 18 days; the original species is generally made with a 500ml can bottle of peat and corncobs, and the cultivation generally takes 45-60 days; the cultivated...

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the defect that the above-mentioned technology exists, provide a kind of molecular identification and bacterial strain production method of the king mushroom, by means of molecular identification, clarify the taxonomic status of the mushroom system; by changing the growth environment of the mushroom Conditions, to accelerate the growth rate of the mycelium of Binwang mushroom, shorten the seed production cycle, solve the problem of high pollution rate caused by too long cultivation time in the seed production process of Binwang mushroom, thereby improving the biological transformation rate of Binwang mushroom