Lyophyllum fumosurn molecular identification and strain production method

A technology of molecular identification and production method, which is applied in the field of molecular identification and strain production of Binwang mushroom, can solve the problems of accelerating the growth rate of Binwang mushroom mycelium, high pollution rate of culture time, shortening seed production cycle, etc. The effect of seed success rate and biological conversion rate

Inactive Publication Date: 2015-04-22
LIAONING ACAD OF AGRI SCI
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Problems solved by technology

[0003] The purpose of the present invention is to overcome the defect that the above-mentioned technology exists, provide a kind of molecular identification and bacterial strain production method of the king mushroom, by means of molecular identification, clarify the taxonomic status of the mushroom system; by changing the growth enviro

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Embodiment Construction

[0019] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0020] In 2012, it is expected to produce 2,000 bags of strains of Binwang mushroom. The mother seed will be inoculated on March 5, the liquid seed will be inoculated on March 11, and the cultivated species will be inoculated on March 24. By April 30, the mycelia will be covered with fungus bags. It takes 56 days from the mother seed to the cultivated species, which is more than 50 days shorter than the conventional inoculation method. The pollution rate of the mother species is 0, the pollution rate of the original species is 0, and the pollution rate of the cultivated species is only 3%. The culture medium formula of the cultivated species is used for cultivation Fruiting, biological conversion rate increased from 54% to 60%.

[0021] Through the present invention, the growth rate of the mycelium of the mother species is greatly increa...

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Abstract

The invention discloses a lyophyllum fumosurn molecular identification and strain production method. The method comprises the first step of molecular identification: ITS amplification, product sequencing, Blast isogeny data comparison and classification status determination are carried out; the second step of mother strains: a coconut juice and PDA media component is obtained according to the influence test to the lyophyllum fumosurn mother strain mycelial growth from an exogenous additive and the influence test to the lyophyllum fumosurn mother strain mycelial growth from culture conditions; the third step of stock strain: the liquid media component is screened and studied, the kernel dipping juice, 200 g of potato, 20 g of glucose, 0.3 g of monopotassium phosphate, 0.15 g of magnesium sulfate and 3 g of beef extract are obtained; the fourth step of strain cultivation; the fifth step of the strain production technology; the sixth step of the fruiting media component. The method solves the problem that in the lyophyllum fumosurn strain production process, the contaminate rate is high due to long cultivation time, and therefore the biology converstion rate of the lyophyllum fumosurn is increased.

Description

technical field [0001] The invention belongs to the technical field of edible fungus classification and strain production technology, and relates to a method for molecular identification and strain production of Binwang mushroom. Background technique [0002] The classification of present stage king mushroom is only limited to identification from form and chemical reaction, and conclusion is that king mushroom belongs to Agaricaceae, white mushroom family, and the smoke color of lily mushroom is separated from the genus, and the present invention finds that king mushroom Not only the smoke-colored Limejime, but also the lotus-leaved Limejime, which clarified the taxonomic status of Binwang mushroom; the parent species mycelia of the prior art is overgrown with a 18×180mm test tube slant culture medium, which requires 15~ 18 days; the original species is generally made with a 500ml can bottle of peat and corncobs, and the cultivation generally takes 45-60 days; the cultivated...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
CPCA01G18/00C12Q1/6895
Inventor 刘俊杰宋莹张士义刘岩岩李宏亮刘娜张敏刘国宇李红
Owner LIAONING ACAD OF AGRI SCI
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