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Oocyte culture fluid and culture method thereof

A technology of oocytes and culture methods, applied in the direction of germ cells, animal cells, vertebrate cells, etc., can solve the problems of low success rate, high cost, difficult to promote, etc., to avoid waste, reduce costs, increase cleavage and The effect of chance of blastocyst formation

Inactive Publication Date: 2015-04-22
付蕾
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AI Technical Summary

Problems solved by technology

At present, companies in the world have developed a culture medium system for in vitro maturation of human immature oocytes, and it was commercialized in 2005, but it still cannot get rid of the defects of high cost, low success rate, instability, and difficulty in promotion.

Method used

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  • Oocyte culture fluid and culture method thereof

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Embodiment Construction

[0025] The oocyte culture method of the present invention includes the following steps:

[0026] Patient selection: 138 patients who received ICSI in the Reproductive Medicine Center of Shijiazhuang Fourth Hospital from October 2012 to November 2013, with an average age of (31.56±3.29) years, and the total number of eggs obtained <15 pieces.

[0027] 1. Preparation of human mature follicular fluid: collect the chief follicular fluid from the ovarian corona complex in a conical centrifuge tube, centrifuge at 3000r / min for 10 minutes to remove red blood cells and impurities in the follicular fluid, take 2ml of supernatant and filter Filter by the filter, and place it in a 5ml small test tube in an incubator for equilibrium preservation.

[0028] 2. Preparation of granular cells: Use two 1ml syringes to cut out most of the granular cells around the oocyte under the microscope and place them in a 3037 dish containing 10% HSA-containing G-IVF culture medium and cover with oil. ;

[0029] ...

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Abstract

The invention provides oocyte culture fluid and a culture method of the oocyte culture fluid. The oocyte culture fluid is easy to prepare and low in cost and comprises G-IVF culture fluid, follicular fluid and granular cells. According to the oocyte culture fluid and the culture method of the oocyte culture fluid, the follicular fluid extracted from the follicle of a patient and granular cells around the oocyte are treated specially and added into the culture fluid, so that in-vitro maturity culturing is carried out on the immature egg of the remaining ICSI period, then fertilization, embryonic development and pregnancy results are analyzed, a simple, convenient and effective in-vitro maturity culture scheme is set up, the effective utilization rate of the immature egg is increased, and more pregnancy opportunities can be provided for the infertility patient.

Description

Technical field [0001] The invention relates to a cell culture fluid and a culture method thereof, in particular to an oocyte culture fluid and a culture method thereof. Background technique [0002] For a long time, "infertility" has been a difficult and relatively private matter in people's subconsciousness. With the development of assisted reproductive technology, people gradually understand the in vitro fertilization-embryo transfer technology, and more and more patient couples begin to pay attention to and implement this technology. In vitro fertilization-embryo transfer technology refers to an assisted reproductive technology in which eggs are taken out of the woman's ovary, combined with the man's sperm in a laboratory incubator to form an early embryo, and then transplanted into the woman's uterus. In recent years, in vitro maturation (IVM) of immature oocytes has become a hot spot in the field of reproduction. IVM is a kind of assisted reproductive technology, which is...

Claims

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Application Information

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IPC IPC(8): C12N5/075
Inventor 付蕾周莉袁景川曹琴英葛军
Owner 付蕾
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