A method for identification of resistance to southern rice black-streaked dwarf disease

Abstract

The invention discloses a resistance identification method for southern rice black-streaked dwarf virus variety. The method comprises the steps of (a) acquiring sogatella furcifera, and separating and purifying a large amount of uniform and consistent 5-age sogatella furcifera nymphs; (b) breeding, separating and screening a large amount of uniform and consistent 2-age sogatella furcifera nymphs; (c) feeding virus to the obtained 2-age sogatella furcifera nymphs; (d) calculating the sogatella furcifera nymphs; (e) quantitatively co-breeding rice to be tested and the 2-age sogatella furcifera nymphs fed with virus, and spreading virus; (f) counting land symptomatic appearance, namely, transplanting the rice to be tested to a farm sogatella furcifera separating net chamber, investigating and counting the ratio of infected holes of southern rice black-streaked dwarf virus at the peak tillering phase, so as to accurately identify the resistance of the rice variety to be tested. With the adoption of the resistance identification method, the problems of the interference of antixenosis and the mass acquiring of sogatella furcifera can be overcome; the quantitative and reinforced inoculation identification of southern rice black-streaked dwarf virus can be achieved; the resistance level of the rice variety to southern rice black-streaked dwarf virus can be accurately evaluated.

Description

technical field [0001] The invention relates to a variety resistance identification method for black-streaked dwarf disease of southern rice, which belongs to plant disease identification technology. Background technique [0002] Southern rice black-streaked dwarf disease was first discovered, identified and named by Zhou Guohui and others in Yangxi County, Guangdong Province, my country in 2001. The "black bar dwarf" in the disease name indicates the typical symptoms of the disease, that is, the diseased plant is dwarfed and there are small tumors arranged in black stripes on the surface of the stem. "South" means that the disease was first discovered in South China, and mainly occurred in southern rice areas. Its field symptoms are similar to rice black-streaked dwarf disease, which mainly occurs in cooler wheat-rice growing areas. The rice plants infected with the disease are dwarfed, with no or difficult heading, few and not full grains, and serious loss of yield. Sou...

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Problems solved by technology

[0011] The purpose of the present invention is to provide a southern rice black-streaked dwarf variety for the problem of row-taxis interference in the field natural identification of southern rice black-streaked dwarf and the difficulty of obtaining the scale of white-backed planthoppers for indoor artificial inoculation identification Resistance identification method, this identification method can not only eliminate the interference of row tropism on the identification results, but also solve the problem of obtaining large-scale quantities of white-backed planthoppers required for variety resistance identification, and realize quantitative and enhanced black stripes of southern rice The inoculation identification of dwarf disease finally realized the accurate evaluation of the resistance of southern rice black-streaked dwarf varieties

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