Frictional brushing inoculating method for soybean virus

Abstract

The invention relates to a frictional brushing inoculating method for a soybean virus. The method successively comprises the steps: A, preparation of a virus liquid: planting a soybean variety: Nannong 1138-2, the resistance of which to the soybean virus is susceptible and then collecting diseased leaves infected with the soybean virus on a plant; the putting the diseased leaves, a phosphate buffer and carborundum in a mass ratio of 1: (5-10): (0.4-0.6) in a mortar to be ground and homogenized to obtain a virus liquid, and freezing the virus liquid at 80 DEG C below zero for later use; B, soybean seedling growing: growing the soybean seeds, wherein a sterilizer is not used in growing the seedlings; and obtaining the seedlings for inoculation when the seedlings grow till a pair of main leaves are folded; and C, virus inoculation: disinfecting a soft-bristle toothbrush, then dipping the virus liquid with the soft-bristle toothbrush, and then frictionally brushing the leaves of the seedlings for inoculation with the soft-bristle toothbrush for 2-3 times; and then, spraying clean water to eliminate a residual liquid on the leaves. The method is simple to operate and low in miss inoculation rate and can improve the accuracy and reliability for identifying disease resistance in disease-resistant seedling of the soybean virus.

Description

technical field [0001] The present invention relates to the inoculation method of soybean mosaic virus. Background technique [0002] Soybean is the main grain and oil crop in my country. Soybean virus disease is the most harmful disease in soybean production in Sichuan and even in the whole country. Among them, Soybean Mosaic Virus (SMV) is the most common occurrence, which occurs in various soybean production areas, seriously affecting the yield and quality of soybeans; the annual loss of yield is about 10%, and the severe one can reach 50%. %. Because mosaic virus disease can be transmitted by insects such as aphids, there are very few control agents, so cultivating and promoting disease-resistant varieties is the most effective means to control the damage of mosaic virus disease. The disease resistance of mosaic virus disease must be identified in the disease resistance breeding, and the mosaic virus must be inoculated when the disease resistance is identified. The i...

AI Technical Summary

Problems solved by technology

The rubbing and pressing force of hands during inoculation is all based on experience, which is highly random and has large errors. It is easy to miss the inoculation, which will affect the accuracy and reliability of disease resistance identification.

[0005] The inoculation method of the virus-transmitting agent needs to first specially raise non-toxic aphids and other insects as the virus-transmitting agent, and then let the virus-transmitting agent acquire the virus, and then transmit the virus by the virus-transmitting agent; there are many operating procedures and harsh identification conditions. The cost of identification is high; it is difficult to ensure that there is no leakage in the process of virus transmission, and leakage will occur. The accuracy and reliability of disease resistance identification also need to be improved