A kind of inoculation identification method of soybean resistance to whitefly virus disease

Abstract

The invention discloses a method for indoor inoculating and identifying resistance of soybeans for a virus disease of Bemisia tabaci. The method comprises the steps that 1, the large field Bemisia tabaci is obtained, and then a separation and purification are conducted on a plurality of regular Bemisia tabaci imagoes; 2, a plurality of regular third instar Bemisia tabaci nymphs are bred, separated and selected; 3, poison is fed to the obtained third instar Bemisia tabaci nymphs; 4, the Bemisia tabaci nymphs are measured; (e) a to-be-detected soybean variety and the poison third instar Bemisia tabaci nymphs are rationed, fed together and transmitted poison; 5, symptomatic appearance of the large field is counted: the to-be-detected soybeans are transplanted to a large field insect separating hypocaust, investigation and statistics are conducted on morbidity of diseased plants of a soybean Bemisia tabaci virus after the symptomatic appearance of the large field is conducted, and the disease resistance ability of the to-be-detected soybean variety can be accurately identified. According to the method for indoor inoculating and identifying, the interference of antixenosis and the problem of the obtained scale volume of the Bemisia tabaci are overcome, the inoculation and identification of the ration and reinforcement of the soybean Bemisia tabaci virus disease are achieved, and the resistance level of the soybean variety for the soybean Bemisia tabaci virus disease can be accurately evaluated.

Description

technical field [0001] The invention relates to an inoculation identification method of soybean resistance to bemisia tabaci virus disease, belonging to the field of agricultural science and technology. Background technique [0002] Bemisia tabaci, belonging to the order Homoptera, Bemisidae, genus Bemisia, was first reported in 1889 and found on tobacco in Greece, named as Bemisia tabaci. In 1894, the first Bemisia tabaci in the New North District was found on sweet potatoes in Florida, USA, and was identified as Bemisia tabaci. Because of its morphological variability, Bemisia tabaci has produced many synonyms. Therefore, Bemisia tabaci is also known as whitefly and sweet potato whitefly. By 1978, the number of synonyms of Bemisia tabaci had reached 22. Bemisia tabaci has a variety of feeding habits and a wide range of host plants. There are more than 420 species of host plants in 74 families. [0003] Bemisia tabaci sucks the sap of host plants with piercing-sucking mo...

AI Technical Summary

Problems solved by technology

If soybeans with a strong tendency to bemisia tabaci are used as a source of resistance to breed bemisia tabaci virus disease-resistant varieties, large-scale planting will cause bemisia tabaci to be forced to choose to feed the virus, and finally make soybeans resistant to bemisia tabaci virus disease through the field. The disappearance of the false disease resistance manifested by natural disease identification may bring greater losses to agricultural production

[0010] Although the field natural disease investigation method can compare the differences in the traits of Bemisia tabaci among soybean varieties, the identification results are easily affected by the environment, and the scope of application is also limited, and it is impossible to rule out the interference of row tropism, which leads to insufficient identification results precise

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