A large-scale quantitative inoculation identification method for wheat bushy dwarf resistance

A technology of wheat bush dwarf disease and identification method, which is applied in the field of large-scale quantitative and enhanced inoculation identification of wheat bush dwarf disease resistance, and achieves the effect of overcoming large-scale quantity acquisition.

Active Publication Date: 2017-01-11
汶上县泰弘农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to provide a large-scale quantitative enhanced inoculation of resistance to wheat bush dwarf disease in order to solve the problem of interference of tropism in the field natural identification of wheat bush dwarf disease and the problem of obtaining the scale of SBPH in indoor artificial inoculation identification Identification method, this identification method can not only remove the interference of row tropism on the identification results, but also solve the problem of obtaining the scale of SBPH required for the identification of variety resistance, and realize the quantitative and enhanced inoculation identification of wheat bush dwarf disease , and finally achieved an accurate evaluation of the resistance to wheat bush dwarf disease

Method used

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  • A large-scale quantitative inoculation identification method for wheat bushy dwarf resistance
  • A large-scale quantitative inoculation identification method for wheat bushy dwarf resistance
  • A large-scale quantitative inoculation identification method for wheat bushy dwarf resistance

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] In June 2013, the frozen wheat diseased strains collected from the hardest-hit areas were used as the virus source, and this method was used for inoculation identification. During the same period, 1-2 instar non-toxic SBPH nymphs were used as controls, and other treatments were the same. The feeding time was set at 48 hours, and the inoculation intensity was 10 heads / plant. After the symptoms were manifested, the incidence of Cong'ao disease points was investigated.

[0036]

[0037] From the above examples and comparative examples, it can be seen that there is no significant difference in the incidence of bush dwarf disease of the four wheat varieties transmitted by field-sourced SBPH and non-toxic SBPH, indicating that the use of field-sourced SBPH to spread wheat Bush dwarf virus is not disturbed by heteroviruses carried by genetics.

[0038] At present, in the identification of wheat bush dwarf disease, the natural identification method cannot overcome the interf...

Embodiment 2

[0040] In 2013, four wheat varieties were identified by this method, and the four wheat varieties were also identified by the natural disease identification method in the severely affected areas of wheat bush dwarf disease, and the incidence of bush dwarf disease was calculated and the relative resistance of other varieties relative to Qingqing 892 was calculated. Compare.

[0041]

[0042] By comparing the incidence of wheat bush dwarf disease identified by this method with that of natural occurrence in the hardest-hit area, and the relative resistance-sensitivity ratio performance relative to Qingqing 892, it can be found that the results of different varieties of wheat identified by this technology are compared with the relative resistance of natural-onset identification in the hardest-hit area. The ratios are not the same, and the individual varieties have significant differences, which may be caused by the differences in row tendency among wheat varieties.

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Abstract

The invention discloses a large-scale quantitative strengthened inoculation identification method for wheat rosette stunt disease resistance. The method comprises the steps that (a), land laodelphax striatellus is obtained, and a large number of uniform five-year-old laodelphax striatellus nymphs are obtained through separation and purification; (b), a large number of uniform two-year-old laodelphax striatellus nymphs are bred, separated and screened out; (c); poison feeding is carried out on the obtained two-year-old laodelphax striatellus nymphs; (d), the laodelphax striatellus nymphs are calculated; (e), quantitative co-cultivation poison transmission is carried out between wheat varieties to be tested and the positioned two-year-old laodelphax striatellus nymphs; (f), land symptomatic appearance statistics is carried out, wherein wheat to be tested is transplanted to a land insect isolation net room, rosette stunt disease hole incidence is calculated through investigation and statistics in the heading period, and therefore the disease resistance of the wheat varieties to be tested can be accurately identified. According to the large-scale quantitative strengthened inoculation identification method, antixenosis interference is overcome, large-scale laodelphax striatellus is obtained, quantitative strengthened wheat rosette stunt disease inoculation identification is achieved, and the resistance level of the wheat varieties to the wheat rosette stunt disease can be accurately evaluated.

Description

technical field [0001] The invention relates to a large-scale quantitative inoculation identification method for wheat bush dwarf resistance, which belongs to the identification technology of plant disease resistance. Background technique [0002] Wheat is the general designation of Triticum plants, a gramineous plant widely planted all over the world, which originated in the Fertile Crescent region of the Middle East. Wheat is one of the three major grains, most of which are used as food, and only about one-sixth is used as feed. As the second largest food crop in the world, wheat is also the second largest food crop in China, and plays an important role in the food of Chinese people. According to statistics, wheat accounts for about 43% of the total grain consumption in China. In recent years, the world's population has grown and people's living standards have continued to improve, and the demand for food has continued to increase. At the same time, the arable land has g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01K67/033
CPCA01G7/00A01K67/033
Inventor 刘霞
Owner 汶上县泰弘农业科技有限公司
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