A large-scale inoculation identification method for maize rough dwarf resistance

A technology of maize rough shrinkage disease and identification method, which is applied in the fields of horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems such as the tendency of expulsion to interfere with the acquisition of the large-scale quantity of S. streus Effect

Active Publication Date: 2017-10-31
恒基利马格兰种业有限公司
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Problems solved by technology

[0011] The purpose of the present invention is to provide a large-scale inoculation identification method for corn rough dwarf disease resistance in view of the problem of row-taxis interference in the field natural identification of corn rough dwarf disease and the problem of obtaining the scale of SBPH in indoor artificial inoculation identification , the identification method can not only eliminate the interference of rowing tendency on the identification results, but also solve the problem of the scale of SBPH required for the identification of variety resistance, realize the quantitative and intensified inoculation identification of maize rough dwarf disease, and finally realize the Accurate Evaluation of Maize Rough Dwarf Resistance

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  • A large-scale inoculation identification method for maize rough dwarf resistance
  • A large-scale inoculation identification method for maize rough dwarf resistance

Examples

Experimental program
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Embodiment 1

[0035] According to the identification method provided by the present invention, the frozen diseased rice plants taken from the hardest-hit areas are used as the source of the virus, and the nymphs of the first generation of SBPH from the field are inoculated, and the 2nd instar SBPH nymphs are transplanted into the seedlings of different corn varieties after feeding the poison for 48 hours. (Xianyu 335, Nongda 108, Denghai 3622, Jinhai 5, and Zhengdan 958) were inoculated in the insect co-incubation box, the inoculation intensity was 7 heads / plant, and the corn seedlings were sown in the insect-proof net room after 15 days of breeding ; In the same period, 1-2 instar non-toxic SBPH nymphs (from the Zhou Tong Laboratory of Jiangsu Academy of Agricultural Sciences) were used as a comparison example. After 2 days of poisoning, the worms were moved into seedlings of different corn varieties (Xianyu 335, Nongda 108, Denghai 3622, Jinhai 5 and Zhengdan 958) were inoculated in the in...

Embodiment 2

[0040] Example 2 Using this method to identify the incidence of corn rough dwarf disease that occurs naturally in the hardest-hit areas

[0041] Adopt the large-scale enhanced inoculation identification method provided by the present invention, according to the ratio of 10 worms / strain, respectively inoculate corn varieties such as Xianyu 335, Nongda 108, Denghai 3622, Jinhai 5 and Zhengdan 958 with poisonous 2nd instar SBPH The nymphs, the statistical results of field-specific symptoms of the above-mentioned corn varieties to be tested and the statistical results of the incidence of corn rough dwarf disease in the hardest-hit areas during the same period are shown in Table 2.

[0042] Table 2 Statistical table of corn varieties to be tested and the incidence of corn rough dwarf naturally occurring in the hardest-hit areas during the same period

[0043]

Embodiment 3

[0045] Example 3 Differential identification of row tropism of different maize varieties

[0046] In order to verify whether the difference in disease resistance between the results of this technical identification speculated in Example 2 and the natural disease identification in the hardest-hit area is caused by the difference in the row-taxis of different corn varieties, we have done the following experiments on the row-taxis identification of different varieties .

[0047] The corn varieties such as Xianyu 335, Nongda 108, Denghai 3622, Jinhai 5 and Zhengdan 958 were sown on seedling trays respectively, and different corn varieties were sown in the same tray, with 10 plants for each variety in the same tray. A total of 10 trays were planted to replicate. Sow seeds at the first-generation SBPH adult initiation stage and inoculate SBPH at the one-leaf-one-core stage of corn. The seedling trays are placed in a quiet greenhouse for cultivation. The cultivation temperature is c...

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Abstract

The invention discloses a large-scale inoculation identification method for MRDV (Maize Rough Dwarf Virus) resistance. The large-scale inoculation identification method comprises the following steps of (a) obtaining a large amount of small brown rice planthoppers in a field, and separating and purifying a large amount of uniform five-year small brown rice planthopper nymphs; (b) breeding, separating and screening a large amount of uniform two-year small brown rice planthopper nymphs; (c) feeding virus to the obtained two-year small brown rice planthopper nymphs; (d) metering the small brown rice planthopper nymphs; (e) carrying out quantitative co-cultivation virus transmission on to-be-detected maize varieties and the virus-carried two-year small brown rice planthopper nymphs; (f) calculating symptomatic appearance in the field of the to-be-detected maize varieties, wherein the to-be-detected maize varieties are transplanted in a field insect separating net room, and the morbidity situation of MRDV during a maize flowering season is surveyed, so that the MRDV resistance of the to-be-detected maize varieties can be accurately identified. According to the large-scale inoculation identification method disclosed by the invention, the problems of antixenosis interference and large scale of small brown rice planthoppers are overcome, quantitative and strengthened RBSDV (Rice Black Streaked Dwarf Virus) inoculation identification is realized, and the resistance level of rice varieties on the RBSDV can be accurately evaluated.

Description

technical field [0001] The invention relates to a large-scale inoculation identification method for corn rough dwarf disease resistance, belonging to the field of agricultural science and technology. Background technique [0002] Corn is a crop that is used as food, feed and industrial raw materials. my country is the second largest country in the world in corn production, with an annual planting area of ​​about 20 million hectares and an annual output of 75 billion tons. It plays an important role in food safety production. [0003] Maize rough dwarf is a worldwide maize disease caused by maize rough dwarf virus (MRDV). Corn rough dwarf was first found in American corn material grown in Italy in 1949, and it was also found in Israel in the same year. Corn rough dwarf became a pandemic in Europe and the Middle East in the 1960s, and then spread to Argentina in South America. , Brazil and Uruguay, and then spread to North America and Japan, South Korea and China in Asia. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01K67/033
CPCA01G7/00A01K67/033
Inventor 乔保建夏多才贺长刚王云苏浩
Owner 恒基利马格兰种业有限公司
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