Production and use of wheat head blight grain fungus

Abstract

The invention relates to the cultivation of pathogenic bacteria of plant fungal diseases, specifically a method for making and using grain strains of wheat scab, which solves the problem that the existing inoculation identification method needs to cultivate bacterial liquid, the method is complicated, the bacterial liquid cannot be stored for a long time, and the success rate of inoculation is low The preparation of grain strains: soak grains with husks in 5% mung bean water to maintain a water holding rate of 60%, divide them into bags, lightly press them tightly, seal the tubes, sterilize under high temperature and high pressure and let them dry to room temperature, inoculate both ends Cultivate in dark, constant temperature, ventilation and high humidity, transfer to air-permeable paper bags, press in a cool, ventilated place to dry, seal the bags and store at low temperature and avoid light. How to use: First test the activity, inoculate the lemma of the small flowers on the side of the spikelet at the base of the spikelet before flowering after heading, wrap and moisturize until the lemma turns brown, remove the wrapping layer, and use high humidity mist to moisturize for 28 days. Advantages: 1. Strains can be stored for a long time; 2. One-time production, multiple use, less dosage, easy to transport; 3. Simple and efficient inoculation method, high incidence rate, and accurate disease resistance identification results.

Description

technical field [0001] The invention relates to the cultivation of pathogenic bacteria of plant fungal diseases, in particular to methods for making and using wheat scab grain strains. Background technique [0002] Wheat scab (Fusarium head blight, FHB) is a devastating disease caused by Fusarium graminearum (Fusarium graminearum), which seriously damages the ear and grain of wheat. Humid and semi-humid areas such as spring wheat areas. In the year when the disease is prevalent, it can cause 70%-100% yield loss of wheat. Wheat grains infected by Gibberella contain a variety of toxins, especially DON toxin, which is highly toxic to humans and animals, and can cause human and Animal vomiting, diarrhea, skin irritation, food refusal, nervous disorder, miscarriage, stillbirth, etc. When the diseased wheat rate reaches more than 4%, it is inedible and loses its commodity value. [0003] In recent years, with the intensification of global warming and changes in farming systems,...

AI Technical Summary

Problems solved by technology

[0006] The present invention solves the problems that the current inoculation identification method needs to cultivate bacterial liquid, and the preparation method is complicated, the cultivated bacterial liquid cannot be stored for a long time, and the success rate of inoculation is low, and provides a simple preparation method without cultivating bacterial liquid, The prepared grain can be stored for a long time without losing activity, and the production and use method of wheat head blight grain strain with high inoculation success rate