Making and using method of Fusarium head blight grain fungus strains

Abstract

The invention belongs to the field of plant fungous disease pathogen culture, and more specifically discloses a making method and a using method of Fusarium head blight grain fungus strains. The making method is used for solving problems in the conventional fungus inoculation identification method that the method is complex, long time storage of fungus liquid is difficult to realize, and fungus inoculation success rate is low. The making method comprises following steps: grains with husk are immersed in 5% mung bean water to maintain 60% water holding capacity, and are packaged with bags; compacting is carried out, and the bags are sealed to prepare fungus tubes; the fungus tubes are subjected to high temperature high pressure sterilization, and are cooled to room temperature in the air; the two ends of each fungus tube are inoculated with fungus, and culture is carried out at a dark constant-temperature ventilative high humidity environment, and then obtained fungus strains are transferred into ventilative paper bags, are dispersed, are subjected to airing at cold and ventilative places, and are sealed in ziplock bags for low temperature storage in dark. The using method comprisesfollowing steps: activity is detected; after heading before flowering, basal spikelet side floret glumelle is inoculated with the fungus strains, packaging moisture retention is carried out until lemma becomes brown and a packaging layer is removed, and then mist moisture retention is carried out for 28d at high humidity. The advantages are that: 1, the obtained Fusarium head blight grain fungusstrains can be stored for a long term; 2, Fusarium head blight grain fungus strains prepared via one time of preparation can be used in a plurality of times, using amount is low, and transportation isconvenient; and 3, fungus inoculation method is simple, and is high in efficiency, incidence of disease is high, and disease resistance identification results are accurate.

Description

technical field [0001] The invention relates to the cultivation of pathogenic bacteria of plant fungal diseases, in particular to methods for making and using wheat scab grain strains. Background technique [0002] Wheat scab (Fusarium head blight, FHB) is a devastating disease caused by Fusarium graminearum (Fusarium graminearum), which seriously damages the ear and grain of wheat. Humid and semi-humid areas such as spring wheat areas. In the year when the disease is prevalent, it can cause 70%-100% yield loss of wheat. Wheat grains infected by Gibberella contain a variety of toxins, especially DON toxin, which is highly toxic to humans and animals, and can cause human and Animal vomiting, diarrhea, skin irritation, food refusal, nervous disorder, miscarriage, stillbirth, etc. When the diseased wheat rate reaches more than 4%, it is inedible and loses its commodity value. [0003] In recent years, with the intensification of global warming and changes in farming systems,...

AI Technical Summary

Problems solved by technology

[0006] The present invention solves the problems that the current inoculation identification method needs to cultivate bacterial liquid, and the preparation method is complicated, the cultivated bacterial liquid cannot be stored for a long time, and the success rate of inoculation is low, and provides a simple preparation method without cultivating bacterial liquid, The prepared grain can be stored for a long time without losing activity, and the production and use method of wheat head blight grain strain with high inoculation success rate