Method for accurately positioning Bregma point in rat brain stereotaxic surgery

Abstract

The invention discloses a method for accurately positioning a Bregma point in rat brain stereotaxic surgery and application of pasteurization liquid in displaying of the Bregma point. The method comprises the steps of anesthetizing a rat; fixing the head of the rat; disinfecting the head of the rate; cutting off clothing hair; cutting skin of the top part of the head along a central sagittal line through a surgical knife; pulling soft tissue to two sides to expose the sutura position; wiping the sagittal line and the crown line of the head of the rat through a cotton swab with hydrogen peroxide to display a relatively wide crossed strip area; flushing the area with normal saline and wiping; wiping the crossed area with the cotton swab with the pasteurization liquid until the Bregma point is clearly exposed; then flushing with normal saline and wiping again; determining the value of the original point O of a three-dimensional coordinate system of the skull through a brain stereotaxic apparatus. According to the method, the hydrogen peroxide and the pasteurization liquid are used together to corrode calvarium periosteum, which causes little breeding, and the surgical field is clear; two crossed lines of sagittal suture and the crown suture are finely and clearly shown; the Bregma point is clearly exposed; therefore, the accurate positioning is achieved.

Description

technical field [0001] The invention relates to an experimental rat brain stereotaxic technique, in particular to a method for accurately locating Bregma points in experimental rat brain stereotaxic operations, and also relates to the use of pasteurized disinfectant combined with hydrogen peroxide as a rat brain stereotaxic display of Bregma points. Background technique [0002] The stereotaxic technique of experimental rat brain is widely used in the precise positioning of brain damage, stimulation and EEG recording, and has become an indispensable tool for studying brain structure and function. Stereotaxic technique of rat brain mainly uses brain stereotaxic apparatus as a positioning instrument, using some three-dimensional coordinates stipulated by some landmarks outside the skull (such as anterior fontanel, posterior fontanel, external auditory canal, orbit, sagittal suture, etc.) or other reference points System, to determine the location of certain subcortical neural ...

Images