Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Use of λ-carrageenan oligosaccharides in the preparation of anti-rabies virus drugs

A technology of carrageenan oligosaccharide and rabies virus, which is applied in the direction of antiviral agents, can solve the problem of no anti-rabies virus, etc., and achieve the effect of long effective time

Active Publication Date: 2017-04-26
HUAZHONG AGRI UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

λ-carrageenan oligosaccharide has various biological activities such as anticoagulation, antivirus (such as herpes virus), antitumor, etc. However, there is no relevant literature report on antirabies virus at present

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of λ-carrageenan oligosaccharides in the preparation of anti-rabies virus drugs
  • Use of λ-carrageenan oligosaccharides in the preparation of anti-rabies virus drugs
  • Use of λ-carrageenan oligosaccharides in the preparation of anti-rabies virus drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The present invention will be described in detail below in conjunction with examples.

[0021] 1 Materials and methods

[0022] 1.1 Materials

[0023] 1.1.1 Viruses

[0024] Rabies virus laboratory fixed strains SAD-L16, CVS-B2c, rabies virus wild Anhui strain DRV-AH08 (canine rabies virus) are all preserved by the State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University.

[0025] 1.1.2 Marine oligosaccharides

[0026] The λ-carrageenan oligosaccharide was provided by Ocean University of China, with a molecular weight of about 4,000 Da and a maximum water solubility of 50 mg / mL. with DMEM or ddH respectively 2 O was dissolved into a 5mg / mL solution, sterilized by filtration through a 0.22μm microporous filter, and stored at 4°C after aliquoting. Cell growth solution, maintenance solution or ddH was used for experiments. 2 O was diluted to the desired concentration.

[0027] 1.1.3 Cells and monoclonal antibodies

[0028] SK-N-SH cells (h...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses an application of lambda-carrageenan oligosaccharide to preparation of an anti-rabies virus drug. Experiments prove that the lambda-carrageenan oligosaccharide is low in cytotoxicity so that rabies viruses of various strains can be inhibited on various types of cells and inhibition effect is provided to each stage of the rabies viruses invading the cells; and particularly in the stage after virus adsorption, namely, 12 hours after the cells are infected by the viruses, the lambda-carrageenan oligosaccharide is still capable of remarkably decreasing the rabies virus amount in the cells. According to above discovery, the lambda-carrageenan oligosaccharide can be used as a low-cost effective wound treating agent to replace the anti-rabies virus immune globulin, and has the advantages of decreasing the viruses at the wound part to the greatest extent and reducing the rabies morbidity.

Description

technical field [0001] The invention relates to a new drug application of λ-carrageenan oligosaccharide, especially a new application in preparing anti-rabies virus drugs. Background technique [0002] Rabies is one of the oldest infectious diseases and is a zoonotic disease caused by Rabies virus (RABV). Rabies is by far the acute infectious disease with the highest human case fatality rate, once onset, the case fatality rate is almost 100%. According to the report of the World Health Organization (WHO), about 55,000 people die from rabies every year in the world, most of which are distributed in Africa and Asia. In recent years, an average of about 3,000 people died of rabies each year in my country, most of which occurred in vast rural areas or urban-rural fringe areas, and were mainly infected by bites of venomous animals. RABV enters the bite site with saliva, enters the nearest nerve fiber after a brief incubation in the muscle, and gradually migrates to the brain. T...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/737A61P31/14
Inventor 赵凌傅振芳罗召晨周明管华诗王伟
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com