Application of Paenibacillus polymyxa dgnkzx004 in the cultivation of lipstick flower

A technology of Paenibacillus polymyxa, CCTCCM2014504, which is applied in the application field of Paenibacillus polymyxa dgnkzx004 in the cultivation of lipstick, can solve the problems of fast and effective effect, and achieve stable bacteriostatic effect, outstanding effect, and growth-promoting effect. Effect

Active Publication Date: 2017-08-25
DONGGUAN AGRI SCI RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biological control controls pests and diseases through the interaction between organisms, and its effect may not be as fast and effective as chemical pesticides, but their control effect is long-lasting, stable, and environmentally friendly
However, there are no reports and studies on Paenibacillus polymyxa inhibiting flower pathogens and being applied to flower cultivation.

Method used

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  • Application of Paenibacillus polymyxa dgnkzx004 in the cultivation of lipstick flower
  • Application of Paenibacillus polymyxa dgnkzx004 in the cultivation of lipstick flower
  • Application of Paenibacillus polymyxa dgnkzx004 in the cultivation of lipstick flower

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Bacterial strain isolation and identification

[0042] 1. The inventor collected the orchard soil of Dongguan Agricultural Science Research Center on August 2, 2011, weighed 10g of the mixed soil samples at each sampling point, and put them into 100mL sterile water (prepared 1:10 soil suspension) And in the Erlenmeyer flask with glass beads, shake it for about 20 minutes to make the soil sample and water fully mix and disperse, this is the original dilution; then use a 1mL sterile pipette to draw 1mL of the diluted soil solution into a 9mL sterile In the test tube of water, use the suction ear ball to blow and suck several times to mix well, this is 10 -1 Dilute the suspension; by analogy, dilute the soil sample gradient to 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 The soil dilution suspension was repeated 3 times. The prepared dilution was blown and mixed several times, and 10 -2 、10 -4 、10 -6 Use a sterile pipette to draw three gradient dilutions (breat...

Embodiment 2

[0054] Embodiment 2 biological control test

[0055] 1. Cultivation of strains:

[0056] S1. Activation of strains: Put Paenibacillus polymyxa (Paenibacillus polymyxa) dgnkzx004 glycerol strain into 20mL LB liquid medium at the inoculation amount of 2% (v / v), and place it in a 32°C incubator for static activation Cultivate for 20 hours to obtain seed solution;

[0057] S2. Expanded cultivation: Inoculate the activated seed liquid into 1000mL LB liquid medium according to the inoculation amount of 5% (v / v), and culture it statically in a 32°C incubator for 20 hours;

[0058] S3. Preparation of bacterial cell suspension: the fermentation broth was centrifuged to collect the bacterial cells, washed twice with sterile normal saline, centrifuged to obtain the desired viable cells, and finally the bacterial cells were suspended with physiological saline to make a cell suspension.

[0059] Wherein, the centrifugation described in S3 is 9500r / min and 15min at 4°C.

[0060] 2. Prepa...

Embodiment 3

[0071] Embodiment 3 promotes growth test

[0072] 1. The growth-promoting experiment was carried out with the lip safflower as the object

[0073](1) Test material: cutting seedlings were used in the test, and 4 cups with a diameter of 7 cm were inserted. When the new buds grew about 4 to 5 cm and the roots grew to the bottom of the pot, they were transplanted into pots with a diameter of 170 cm for cultivation. Seedlings in 4 small pots 1 large pot, apply 20-20-20 (800-1000 times) once every 4-5 days, and spray the foliage once a day at noon; after 10 days of planting, uniform topping, when the branches grow to 5-6cm, 2nd topping at the same time.

[0074] (2) Experimental grouping

[0075] The cutting seedlings were divided into 3 groups, the compound bacterial agent group, the dgnkzx004 group and the control group (the control group was Huaduoduo general fertilizer 20-20-20).

[0076] Compound bacterial agent group: before cutting, soak the bottom of the cutting seedling...

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Abstract

The invention relates to the field of biotechnology and discloses the application of Paenibacillus polymyxa (Paenibacillus polymyxa) strain dgnkzx004 in the cultivation of lipstick flowers. The strain has been deposited in the China Center for Type Culture Collection (CCTCC) on October 21, 2014, and the deposit number is CCTCC M 2014504. The strain not only has a significant and stable bacteriostatic effect against Corynespora cassiicola, the pathogen of lipstick mosaic leaf spot, but also can promote the growth of lipstick flowers. It has very important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology. More specifically, it relates to the application of Paenibacillus polymyxa dgnkzx004 in the cultivation of lipstick flowers. Background technique [0002] During the growth process of flowers, various natural disasters may be encountered, resulting in poor growth and development of plants, and even dim color of plants, withered leaves, flower rot, deformed organs, and dry stems, thereby reducing the yield and quality of flowers and causing serious economic losses. . The main causes of flower diseases are environmental reasons and pests and diseases. Environmental reasons mainly cause physiological diseases, which are caused by unsuitable climate and soil conditions. Frequent physiological diseases include burns caused by strong light in summer, freezing damage caused by low temperature in winter, root rot caused by excessive water, and leaves caused by insufficient water. Scorched edges, wilting, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P3/00A01P21/00
Inventor 徐匆郑芝波
Owner DONGGUAN AGRI SCI RES CENT
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