Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Xu Changqing c21 steroid conversion product and its application

A transformation product, Xu Changqing's technology, applied in the field of medicine, can solve the problems of no high content, no research reports on tumor cell multidrug resistance reversal agents, etc.

Inactive Publication Date: 2018-01-26
QINGDAO UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been reports in the literature on the isolation and identification of C21 steroidal compounds from Xu Changqing, but there is no product with a high content of Xu Changqing's C21 steroid conversion product; there is no information about the use of Xu Changqing's C21 steroid conversion product in the preparation of tumor cell multidrug resistance reversal agents research report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Xu Changqing c21 steroid conversion product and its application
  • Xu Changqing c21 steroid conversion product and its application
  • Xu Changqing c21 steroid conversion product and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Method for Determination of C21 Steroid Aglycon Weight Percentage in Xu Changqing C21 Steroid Transformation Products by Colorimetry

[0038] Solution preparation

[0039] Preparation of reference solution

[0040] Accurately weigh an appropriate amount of albino aglycone C reference substance, add methanol to make each 1 ml containing 186.32 mu g solution, as the reference solution.

[0041] Preparation of the test solution

[0042] Take 0.1 g of Xu Changqing C21 steroid conversion product, accurately weigh it, add methanol to dissolve, transfer to a 50 ml measuring bottle, add methanol to the mark, shake well, accurately draw 10 ml, put it in a 100 ml measuring bottle, add methanol to the mark , Shake well, filter, and take the continued filtrate as the test solution.

[0043] Assay method

[0044] Precisely draw the test solution 100 mu l, put it in a stoppered test tube, evaporate the solvent, add 5 ml of freshly prepared concentrated sulfuric acid-methanol ...

Embodiment 2

[0046] Take 300 Kg of dried root and rhizome powder of Xu Changqing (produced in Shandong), add 90% ethanol to reflux and extract three times, each time the dosage is 2400 L, each extraction time is 2 hours, filter in several times, the extracts are combined, and recovered under reduced pressure Ethanol, the obtained extract was dissolved in water, chromatographed on a macroporous adsorption resin column D101, filled with a macroporous adsorption resin D101 to a volume of 150 L, eluted with water until the eluate was nearly colorless, discarded the eluent, and washed with 30% ethanol Elute until the eluent is nearly colorless, discard the eluent, elute with 70% ethanol until the eluent is nearly colorless, collect the eluate, and perform column chromatography on the decolorized resin D941 that has been treated, fill the volume of decolorized resin D941 150 L, elute with 900 L of 90% ethanol, collect the eluate, concentrate under reduced pressure, and dry to obtain the eluate, a...

Embodiment 3

[0048]Take 300 Kg of dried root and rhizome powder of Xu Changqing (produced in Guangxi), add 90% ethanol to reflux and extract three times, each time the dosage is 2400 L, each extraction time is 2 hours, filter in several times, the extracts are combined, and recovered under reduced pressure Ethanol, the obtained extract was dissolved in water, chromatographed on a macroporous adsorption resin column D101, filled with a macroporous adsorption resin D101 to a volume of 150 L, eluted with water until the eluate was nearly colorless, discarded the eluent, and washed with 30% ethanol Elute until the eluent is nearly colorless, discard the eluent, elute with 70% ethanol until the eluent is nearly colorless, collect the eluate, and perform column chromatography on the decolorized resin D941 that has been treated, fill the volume of decolorized resin D941 150 L, elute with 900 L of 90% ethanol, collect the eluate, concentrate under reduced pressure, and dry to obtain the eluate, add...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a Xu Changqing C21 steroid transformation product and its application in the preparation of a multidrug resistance reversal agent for tumor cells. The weight percentage of C21 steroid aglycone in the transformation product is greater than or equal to 90%, which can reverse the multidrug resistance of human gastric cancer. Drug-resistant cells, multidrug-resistant cells of human colon cancer, and multidrug-resistant cells of human lung cancer are resistant to commonly used clinical drugs paclitaxel, doxorubicin, 5-fluorouracil, and cisplatin, and also provide preparation of Xu Changqing's C21 steroid transformation product The method and the preparation method are simple and convenient. The Xu Changqing C21 steroid transformation product of the present invention is used in combination with antineoplastic drugs in clinic to improve the curative effect of chemotherapy and has broad application prospects.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to Xu Changqing's C21 steroid conversion product and its application in preparing tumor cell multidrug resistance reversal agents, and also relates to a preparation method of Xu Changqing's C21 steroid conversion product. Background technique [0002] Malignant tumor is one of the diseases that seriously endanger human health. It is characterized by abnormal cell proliferation and metastasis. Statistics from the World Health Organization show that in 2000, there were about 10 million new cases of malignant tumors in the world, 6.2 million deaths, and 22 million cases of illness; it is estimated that by 2020, there will be 15 million new cases of malignant tumors, 10 million deaths, and 30 million illnesses. Thousands of cases. Chemotherapy is one of the methods of comprehensive therapy for malignant tumors. It is widely used before, during and after surgery. However, the phenomenon of multidr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/27A61P35/00A61K125/00
Inventor 王威刘小红褚文希高华韩立春
Owner QINGDAO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com