Chloral hydrate permeabilization flaking method in microscopic identification of TCM

Abstract

The invention relates to a chloral hydrate permeabilization flaking method in microscopic identification of TC, which can effectively solve the problem of easy control, high efficiency and safety of chloral hydrate permeabilization. The method comprises the following steps: adding water in chloral hydrate for dissolving to obtain a chloral hydrate solution with mass concentration of 30-50%; adding 0.1-0.5g of traditional Chinese medicinal material powder into 2ml of chloral hydrate solution with mass concentration of 30-50% to prepare a mixture; filling the mixture into a container, preheating to 100-110 DEG C, then heating to 125-135 DEG C for 4-8 minutes, realizing permeabilization of the mixture; dropping the 1-2 drops of permeabilized mixture on a slide glass, adding 1-2 drops of dilute glycerin, uniformly mixing the traditional Chinese medicinal material powder on the slide glass, covering a coverslip, using an absorbent paper to remove overflowed liquid, and keeping the clean coverslip and slide glass. The flaking method can be effectively used for chloral hydrate permeabilization, and has the advantages of energy saving, safety, high efficiency, controllable performance, wide application and environmental protection, is especially suitable for teaching and scientific research, has strong utility value, and is great innovation of chloral hydrate permeabilization flaking method.

Description

technical field [0001] The invention relates to the quality detection of traditional Chinese medicines, in particular to a method for permeabilizing and preparing tablets of chloral hydrate in traditional Chinese medicines for microscopic identification. Background technique [0002] Permeabilization of aldehyde hydrate into tablets is a common technical operation in the identification methods of traditional Chinese medicines, and the quality of the preparation directly affects the identification. However, with the development needs of teaching and scientific research, the shortcomings of this traditional permeabilization method gradually emerged. In addition to the danger of open flames in the use of alcohol lamps in the test, uneven heating and generation of Bubbles, scorched powder, etc., greatly affect the accuracy and efficiency of the experiment. [0003] The chloral hydrate permeabilization method is the most commonly used material processing method in the microscopi...

AI Technical Summary

Problems solved by technology

However, in the long-term practice, the shortcomings of this traditional permeable method have gradually emerged.

The use of alcohol lamps in the experiment has the danger of open flames, the temperature is difficult to control during film production, uneven heating is likely to occur, the powder is scorched, easy to generate air bubbles, and the flame of the alcohol lamp is easy to blacken the glass slides, etc., which greatly affects the microscope. clear observation of form

Chloral hydrate emits toxic gas when heated, is poisonous and irritating

Especially in the experimental teaching of Chinese medicine colleges and universities, if many people use it frequently in a poorly ventilated classroom for a long time, it will cause harm to teachers and students, and it is not easy to control, low in efficiency, and poor in safety.

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