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Determination method for the content of gastrodin in Gastrodia elata

A determination method, gastrodin technology, applied in the field of chemical detection, can solve the problems of long test time, high skill requirements of testers, large amount of solvent, etc., and achieve the effect of less solvent amount, clear detection accuracy, and high measurement accuracy

Active Publication Date: 2016-08-10
广西芊谷生源农业发展有限公司
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  • Claims
  • Application Information

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Problems solved by technology

The above method uses a large amount of solvent, takes a long time to test, and requires high skills of testers

Method used

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  • Determination method for the content of gastrodin in Gastrodia elata
  • Determination method for the content of gastrodin in Gastrodia elata
  • Determination method for the content of gastrodin in Gastrodia elata

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Embodiment 1

[0025] The instruments and consumables used in this embodiment are: Pulverizer, Electronic Analytical Balance (XA205DU): Sensitivity 0.001g, Accelerated Solvent Extractor Model: ASE-350 (10ml extraction pool, 50ml collection bottle), volumetric bottle: 25ml, Centrifuge tube: 2ml.

[0026] Step 1: Take about 1g of Gastrodia elata powder (passed through a No. 3 sieve), weigh it accurately, mix it with 1g of diatomaceous earth, and set aside;

[0027] Step 2: Pad 3 to 6 sheets (1 piece of commercial filter paper) at the bottom of the extraction pool with homemade circular filter paper (diameter 27mm), add about 1g of diatomaceous earth, then add the homogeneously mixed sample obtained in step 1, and then add an appropriate amount of diatomaceous earth , shake gently to make it at the same level as the pool mouth, and tighten the upper cover of the extraction pool.

[0028] Step 3: extract and extract with ASE to obtain the extract, the specific parameters are shown in Table 1 be...

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Abstract

The invention discloses a method for determining the content of gastrodin in gastrodia elata, which comprises the following steps: step 1: crush gastrodia elata and mix it with diatomite, the weight ratio of gastrodia elata and diatomite is 1:1, and the gastrodia elata after being crushed The particle size is Gastrodia elata powder sieved by No. 3 sieve; step 2: extract gastrodin by ASE extraction method, and collect the extract, wherein the extraction solvent used in the ASE extraction method is dilute ethanol with a volume fraction of 49.5-50.5% solution; step 3: after evaporating the extract to dryness, dissolve it with a mixture of acetonitrile and 0.05% phosphoric acid aqueous solution and dilute to constant volume, and get the supernatant after centrifugation; step 4: detect the above supernatant with high performance liquid chromatography Determination of gastrodin content. The purpose of the present invention is to provide a method for determining the content of gastrodin in Gastrodia elata with rapid extraction and high measurement accuracy.

Description

technical field [0001] The invention relates to the technical field of chemical detection, in particular to a method for determining the content of gastrodin in Gastrodia elata. Background technique [0002] Gastrodia elata is the dry tuber of the orchid plant Gastrodiaelata B1. It is excavated from the beginning of winter to before Qingming of the next year, washed immediately, steamed thoroughly, and dried at low temperature in the open. Gastrodia elata is oval or strip-shaped, slightly flat, shrunken and slightly curved, 3-15cm long, 1.5-6cm wide, and 0.5-2cm thick. The surface is yellowish-white to light yellowish-brown, with longitudinal wrinkles and multiple rounds of horizontal rings arranged by latent buds, and sometimes tan fungal cords can be seen. There are reddish-brown to dark brown parrot-shaped buds or residual stem bases at the top; there is a round navel-shaped scar at the other end. The texture is hard, not easy to break, the section is relatively flat, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 陈学松陈翠玲
Owner 广西芊谷生源农业发展有限公司
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