Preparation method of up-conversion nanocrystal-hypocretin complex and application of the prepared complex in photosensitization therapy

A technology of nanocrystals and oleocanthin, applied in the field of photosensitizers, can solve the problems of weak light source tissue penetration ability, inability to achieve tumor treatment, etc., and achieve the effects of low dark toxicity, long excitation wavelength, and high phototoxicity

Inactive Publication Date: 2018-02-09
NANJING NORMAL UNIVERSITY
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Problems solved by technology

Taking advantage of this property, the development of upconverting NaYF 4 :Yb 3+ ,Tm 3+ The nanocrystal-hypocretin complex can overcome the difficulty that hypocrellin excites the light source tissue penetration ability and cannot realize the treatment of solid tumors in vivo

Method used

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  • Preparation method of up-conversion nanocrystal-hypocretin complex and application of the prepared complex in photosensitization therapy
  • Preparation method of up-conversion nanocrystal-hypocretin complex and application of the prepared complex in photosensitization therapy
  • Preparation method of up-conversion nanocrystal-hypocretin complex and application of the prepared complex in photosensitization therapy

Examples

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Embodiment 1

[0030] Take a 50mL three-necked flask, add 1mmol of Ln(CF 3 COO) 3 (Y:Yb:Tm=78%:20%:2%), 10mmol sodium fluoride (NaF) and 20ml organic solvent (3ml oleic acid / 17ml octadecene), heated to 160°C, in an anhydrous and oxygen-free atmosphere Continue heating in medium for 1h. Then the temperature was raised to 320° C. and maintained for 1 h while magnetic stirring, and naturally cooled to room temperature after the reaction was completed. Add absolute ethanol to the obtained product, obtain a precipitate after centrifugation, and repeatedly wash with water and ethanol three times, then wash the product in hydrochloric acid (pH=4.0), and extract with a mixed solvent of water / ether to obtain a surface free of oleic acid Modified, pure, upconverting NaYF that can be stably dispersed in aqueous phase 4 :Yb 3+ ,Tm 3+ nanocrystals. Then, add 200 uL HA solution (15mM, DMSO as a solubilizer) into the system, stir magnetically at room temperature for 24 hours, and use twice distilled...

Embodiment 2

[0039] Embodiment 2 is basically the same as Example 1, but the hypocrellin A is replaced by hypocrellin B. upconverting NaYF 4 :Yb 3+ ,Tm 3+ The nanocrystal-hypocretin complex was characterized by the same method as in Example 1, and the results were basically similar.

Embodiment 3

[0040] Example 3 is basically the same as Example 1, and the mass ratio of the nanocrystals to hypocretin is 1:0.1˜1:10.

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Abstract

A preparation method for upconversion NaYF4: yb3+, Tm3+ nanocrystals-hypocretin complex, using a hydrothermal method to synthesize upconversion NaYF4: yb3+, Tm3+ nanocrystals with an excitation wavelength of 980nm and an upconversion fluorescence wavelength of 470nm, and removing them under acidic conditions The surface modifier of the nanocrystal, obtain the nanocrystal aqueous solution, add the hypocretin solution, incubate, dialyze to remove the unadsorbed hypocrellin and solvent, and obtain the nanocrystal-hypocretin composite thing. The method of the present invention loads hypocrellin on the surface of up-conversion nano crystals to form a complex, so as to achieve the purpose of long-wave sensitization of hypocrellin, and overcomes the shortcomings of short hypocretin excitation wavelength and shallow tissue penetration depth of the treatment light source. Thereby promoting its application in the treatment of solid tumors in vivo. The invention also relates to the application of the complex prepared by the method in the preparation of photosensitive therapeutic drugs.

Description

technical field [0001] The invention belongs to the technical field of photosensitizers with photodynamic activity, and relates to a long-wave sensitized up-conversion NaYF 4 :Yb 3+ ,Tm 3+ Preparation method of nanocrystal-hypocretin complex and application of the prepared complex in photosensitization therapy. Background technique [0002] Photodynamic therapy (PDT), also known as photoradiation therapy or photochemotherapy, is a new type of tumor treatment. The principle is to use the light source to sensitize the phototherapeutic anticancer drug at the tumor site to undergo a photochemical reaction to generate reactive oxygen species to induce tumor damage. In the process of photodynamic therapy to treat tumors, the key factor is to select phototherapeutic drugs with excellent reactive oxygen generation ability. So far, the antitumor activity of various phototherapeutic drugs such as hematoporphyrin derivatives, metallophthalocyanines, chlorin, 5-aminolevulinic acid a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61P35/00
Inventor 周林周家宏魏少华袁秀雪肖梦思
Owner NANJING NORMAL UNIVERSITY
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