Nucleic Acid Amplification Reaction Apparatus

Abstract

A nucleic acid amplification reaction apparatus includes a mounting section that mounts a nucleic acid amplification reaction vessel including a first side wall, and a second side wall that is arranged opposite to the first side wall, a distance between an inner surface of the first side wall and an inner surface of the second side wall is a length in which a nucleic acid amplification reaction solution comes into contact with both the first side wall and the second side wall, a first heating section that heats a first region of the nucleic acid amplification reaction vessel, a fluorescence measurement unit that measures fluorescence radiated by emitting excitation light to the side wall of the nucleic acid amplification reaction vessel, and a driving mechanism that switches arrangement of the mounting section, the first region, and the second region into a first arrangement or a second arrangement.

Description

technical field [0001] The invention relates to a nucleic acid amplification reaction device. Background technique [0002] As a method for rapidly amplifying nucleic acid, a method is known in which oil and a small amount of nucleic acid amplification reaction solution are put into a cylindrical nucleic acid amplification reaction container, one end of the container is maintained at a high temperature, and the other end is maintained at a high temperature. When the temperature is low, the container is rotated to alternately switch between a state where the reaction liquid is placed in high-temperature oil with one end of the container vertically downward, and a state where the reaction liquid is located in low-temperature oil with the other end vertically downward. The state in the medium, so that the temperature of the nucleic acid amplification reaction solution rapidly produces a thermal cycle (for example, refer to Citation 1). In the nucleic acid amplification reactio...

AI Technical Summary

Problems solved by technology

If the management of the heating amount of the nucleic acid amplification reaction solution is not adequately performed, there is a possibility that a difference may occur in the amount of nucleic acid amplification

In addition, the drop position and the focus position of the detection mechanism tend to deviate, making it difficult to stably detect the amount of nucleic acid.

Furthermore, since the sides of the cylindrical reaction container are curved, if excitation light is irradiated toward the side of the reaction container, the light will be reflected on the surface of the reaction container.

Therefore, it becomes difficult to stably detect the amount of nucleic acid in the reaction container via the side of the reaction container.

On the other hand, even if excitation light is irradiated to the reaction liquid in the reaction container through the bottom of the cartridge, when the light-shielding substance mixed in the reaction liquid accumulates on the bottom of the reaction container, it may become difficult to stably detect the amount of nucleic acid.

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