A method of using pseudobulbs to induce plant regeneration and propagation

Abstract

The invention belongs to a rapid propagation method for important plant materials in rare and endangered wild plants, and particularly relates to a wild orchid tissue culture method. The method comprises material selection and disinfection, induction culture, propagation culture, root induction and transplant. The invention aims to provide a method by adopting pseudobulb to induce regeneration and propagation of malaxis microtatantha plants, and to provide technical support for promoting the study and application of rare orchid species. The method is high in propagation coefficient and high in rooting percentage; plants are short in growth time, good in growth and tidy; the seedling cultivation process is greatly shortened, and the cost is significantly lowered. The method also provides technical support for malaxis microtatantha protection as well as large-scale and industrialized production, popularization and application.

Description

technical field [0001] The invention belongs to a method for rapid propagation of important plant materials in endangered wild plants, in particular to a tissue culture method for wild orchids. Background technique [0002] Konumoran M. microtatantha (Schlechter) Szlachetko , Orchidaceae, a species endemic to China, a national second-class protected plant (CITES II), ground-growing small herb, small pseudobulbs, ovate or nearly spherical, 1 leaf, close to the ground, ovate to broadly ovate, flowers Scapes erect, slender, often purple, slightly compressed, with very narrow wings on both sides; racemes 1-2 cm long, usually with 10-20 flowers, small yellow flowers, flowering in April, distributed in Jiangxi Central (Donggu), Fujian (Wuyishan, Yongfu, Lianjiang) and eastern Taiwan (Taroko). Born in forests or on rocks in damp places, 200–600m above sea level. The symbiosis with moss has very strict requirements on the environmental conditions. It is generally distributed in ...

AI Technical Summary

Problems solved by technology

In view of the small size of the plant, if it is not in the flowering stage, it is difficult to identify, and there are also problems such as natural reproduction ability, weak propagation ability, and difficulty in natural renewal, resulting in the scarcity of excellent seedlings

The plant regeneration of Orchid chrysalis helps to solve the shortage of resources, relieve the pressure of severe damage to wild resources, and effectively protect the limited wild resources to provide technical support for promoting the research and utilization of rare orchid varieties, and to induce There is no report on the regeneration of bulbous plants of Orchid chinensis