Fasting levels of growth hormone as a predictive marker of cardiovascular risk

A growth hormone and cardiovascular technology, applied in biological testing, biomaterial analysis, measuring devices, etc., can solve problems such as low hGH concentration, hindering accurate interpretation of plasma hGH concentration, and undetectable

Active Publication Date: 2018-01-19
SPHINGOTEC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Accurate interpretation of plasma hGH concentrations is hampered by the fact that hGH is released in pulses for most of the day, and during the morning hours when plasma concentrations are relatively stable {32},{33},{34}, using regular hGH Measured hGH concentration is low and sometimes undetectable

Method used

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  • Fasting levels of growth hormone as a predictive marker of cardiovascular risk
  • Fasting levels of growth hormone as a predictive marker of cardiovascular risk
  • Fasting levels of growth hormone as a predictive marker of cardiovascular risk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Embodiment 1, us-hGH assay

[0105] Chemicals

[0106] Chemicals were obtained in p.a. grade from Merck (Darmstadt, Germany) if not stated otherwise.

[0107] antigen

[0108] We used recombinant human growth hormone (NIBSC code 98 / 574, National Institute for Biological Standards and Control, Herfordshire, UK) for immunization and for calibration.

[0109] Antibody Development

[0110] A mouse monoclonal antibody against hGH was developed by UNICUS (Karlsburg, Germany).

[0111] The antibodies were produced according to the following method:

[0112] BALB / c mice were immunized with 100 μg hGH (emulsified in 100 μl complete Freund’s adjuvant) on days 0 and 14, and 50 μg (in 100 μl incomplete Freund’s adjuvant) on days 21 and 28. agent). Three days before fusion experiments, animals received 50 μg of the conjugate dissolved in 100 μl of saline, given as an intraperitoneal and an intravenous injection.

[0113] Splenocytes from immunized mice and cells of the myelo...

Embodiment 2

[0131] Example 2 Population Research

[0132] Subject and Method

[0133] research group

[0134] Malmö The Diet and Cancer Study (MDC) is a population-based, prospective epidemiological cohort (cohort) of 28449 individuals examined between 1991 and 1996 {18}. A random sample from this cohort, examined between November 1991 and February 1994 (n=6103), was included in the MDC Cardiovascular Cohort (MDC-CC), with the primary purpose of studying carotid Epidemiology of Disease {19}. When excluding individuals lacking fasted plasma sample values ​​and thus missing diabetes prevalence data or fasted values ​​for HDL-C, LDL-C or hGH, 4453 individuals remained and were included in the main study cohort.

[0135] All participants provided written informed consent and the study was approved by the ethics committee of Lund University, Lund, Sweden.

[0136] Clinical Examination and Measurements

[0137] Participants underwent medical history, physical examination, and laboratory ...

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Abstract

The subject of the present invention is a method of predicting the cardiovascular risk or the risk of total death in a subject, comprising: determining the inhibition of growth hormone (hGH) and / or its isoforms in body fluids obtained from said subject and · correlating fasted levels of said growth hormone (hGH) and / or isoforms thereof with cardiovascular risk or overall mortality risk, wherein increased levels predict increased risk.

Description

field of invention [0001] The subject of the present invention is a method of predicting the cardiovascular risk or the overall risk of death in a subject, comprising: [0002] Determining fasted levels of growth hormone (hGH) and / or isoforms thereof in body fluids obtained from said subject; and [0003] • Correlating fasted levels of the growth hormone (hGH) and / or isoforms thereof with cardiovascular risk or overall risk of death, wherein increased levels predict increased risk. Background of the invention [0004] Growth hormone (hGH) is secreted from the anterior pituitary gland and many, but not all, of its metabolic effects are exerted through IGF-1, {1}. In addition to regulating longitudinal bone growth during childhood and adolescence, hGH exerts metabolic effects throughout life, such as in the anabolic state, acting with insulin to stimulate IGF-1 leading to protein synthesis and anabolism. In the catabolic state, hGH stimulates lipolysis and thereby prevents p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74
CPCG01N33/74G01N2333/61G01N2800/32G01N33/53G01N2800/2871G01N2800/324
Inventor 安德里亚斯·伯格曼
Owner SPHINGOTEC
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