Hybridoma cell strain 2C1 capable of secreting CAV-2 monoclonal antibodies, monoclonal antibodies secreted by cell strain and application
A hybridoma cell line, CAV-2 technology, applied in the field of monoclonal antibodies
Inactive Publication Date: 2015-10-07
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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At present, there is no report on the development of monoclonal antibody against CAV-2 and its diagnostic colloidal gold test strip
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[0036] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention. within the scope of protection.
[0037] The invention provides a method for preparing anti-CAV-2 monoclonal antibodies that recognize different epitopes, the specific contents are as follows:
[0038] 1 Materials and methods
[0039] 1.1 Materials, experimental animals and reagents
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The invention discloses a hybridoma cell strain 2C1 capable of secreting CAV-2 monoclonal antibodies, the monoclonal antibodies secreted by the cell strain and application. The preservation number of the hybridoma cell strain 2C1 is CGMCC No. 10878. An indirect immunofluorescence result shows that the monoclonal antibodies secreted by the hybridoma cell strain 2C1 can specifically recognize CAV-2 viruses and do not react with MDCK (madin-darby canine kidney) cells. A result of a superposition experiment of the hybridoma cell strain 2C1 and two monoclonal antibodies secreted by another hybridoma cell strain shows that the two monoclonal antibodies are used for different antigenic epitopes of the CAV-2 viruses. The monoclonal antibody prepared by the invention can react with CAV-2 well and can be used for identifying a clinic isolated strain. The 2C1 monoclonal antibody and the 7D7 monoclonal antibody which are prepared by the invention are used for recognizing two different antigen determinants of the CAV-2, so that the monoclonal antibodies can be used for detecting preparation of CAV-2 pathogenic colloidal gold and establishment of a sandwiched ELISA (enzyme-linked immuno sorbent assay) method.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to a hybridoma cell line secreting CAV-2 monoclonal antibody, the monoclonal antibody secreted by the cell line and application thereof. Background technique [0002] Adenovirus (Canln adenovll.us, CAV) is one of the viruses with the most detailed studies on biological characteristics such as morphological structure and gene replication mechanism. The capsid of adenovirus is composed of three main structural proteins: fibrils, hexons and pentons. It has strong infectivity, clear morphological structure, and is widely used in scientific research. Currently, the known serotypes of canine adenoviruses are type 1 and type 2. Although there is immune cross-protection between the two, they are different in pathogenic tropism. The homology of the adenovirus genomes of these two serotypes is between 57-70%. Canine adenovirus infection is a viral infectious disease of dogs, which mainly causes h...
Claims
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Login to View More IPC IPC(8): C12N5/20C07K16/08G01N33/577G01N33/569
Inventor 蔡雪辉王淑杰刘鹤刘永刚
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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