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Preparation method of low-concentration KT (kinetin) gel

A technology of KT gel and preparation method, applied in the field of plant biology, can solve the problems of loss of branches, high cost, unsatisfactory production effect, etc., and achieve the effects of reducing water evaporation and easy operation.

Inactive Publication Date: 2015-11-04
钱家静
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment

[0011] A preparation method of low-concentration KT gel, characterized in that, the method includes the preparation of hormone mother solution, the preparation of working solution, and the preparation of hormone gel, and the specific operation steps are as follows:

[0012] ① Hormone mother solution preparation: Accurately weigh 0.1g of KT and dissolve it with a little 1M KOH, then dilute to 100mL with distilled water, which is 1mg / mL KT mother solution;

[0013] ②Preparation of working solution: Dilute the above mother solution according to the volume ratio of 1:100 into a low-concentration hormone working solution with a concentration of 10mg / L;

[0014] ③ Hormone gel preparation: Accurately weigh 1 g of sodium carboxymethylcellulose and 8 g of sodium alginate, and dissolve them in 100 mL of hormone working solution with a concentration of 10 mg / L in a water bath at 50 °C for later use.

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Abstract

The invention discloses a preparation method of low-concentration KT (kinetin) gel and belongs to the field of plant biotechnology. Firstly, a KT mother solution with the concentration being 1 mg / mL is prepared and diluted to form a low-concentration KT working solution with the concentration being 10 mg / L, and a low-concentration KT gel confining liquid is prepared by dissolving sodium carboxymethylcellulose with the mass concentration being 1% and sodium alginate with the concentration being 8% in the working solution.

Description

Technical field [0001] A low -concentration KT gel preparation method belongs to the field of plant biotechnology. Background technique [0002] Cutting reproduction is one of the common methods of plants, especially trees without any sexual reproduction. This method has many advantages such as low cost, fast -effectiveness, short period of time, short period of time, rich sources of branches used, and can maintain the excellent characteristics of the pro -proof.Different types of hormones are often used during cuttings, such as IAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, ethylene, B 9 , PP333, MH, etc., the addition of the above hormones will enable plants to take root, flowering, fruiting, etc. under the condition of body or departure, increase the number of roots, induces the axillary buds, increase the fruit rate, etc., and play in agricultural production.Mainly useful use.However, it is extremely easy to cause death due to long -term losses of water or nutrients duri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N43/90A01P21/00A01G7/06A01G1/00
Inventor 钱家静
Owner 钱家静